PMID- 21663767
OWN - NLM
STAT- MEDLINE
DCOM- 20111121
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 176
IP  - 1-2
DP  - 2011 Sep
TI  - Development and preliminary application of an immunochromatographic strip for 
      rapid detection of infection with porcine reproductive and respiratory syndrome 
      virus in swine.
PG  - 46-52
LID - 10.1016/j.jviromet.2011.05.034 [doi]
AB  - A "strip test" to detect porcine reproductive and respiratory syndrome virus 
      (PRRSV) was established using a monoclonal antibody (MAb) 2D7 conjugated with 
      colloidal gold. Two MAbs binding to protein N at different epitopes, 2D7 and 1G7 
      were obtained. In the test, samples of PRRSV bound to colloidal gold-conjugated 
      MAb 2D7. The complex was then captured by MAb 1G7 at the test line (T) on the 
      nitrocellulose membrane, presenting a purple band. If the sample did not contain 
      PRRSV or if the quantity of PRRSV was less than that required for the kit, only 
      the control line (C), in which goat anti-mouse antibody was added as the capture 
      antibody, was present. Results from the sensitivity test of the kit demonstrated 
      that the lowest detected quantity of PRRSV is 2.9 x 10(3)TCID(50)/ml. In clinical 
      trials, the specificity and the sensitivity of this kit are 98.1% and 88.4%, 
      respectively, compared with RT-PCR. Furthermore, this kit was found to be 
      efficient in three areas of China and appears to have better results in practical 
      applications than in empirical studies. In summary, this kit has not only high 
      rates of specificity and sensitivity but also has the beneficial features such as 
      efficiency, convenience and speed.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Li, Xue-song
AU  - Li XS
AD  - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, No 427 Maduan St., Nangang District, Harbin 150001, China.
FAU - Fu, Fang
AU  - Fu F
FAU - Lang, Yue-kun
AU  - Lang YK
FAU - Li, Hai-zhong
AU  - Li HZ
FAU - Wang, Wei
AU  - Wang W
FAU - Chen, Xin
AU  - Chen X
FAU - Tian, Hua-bin
AU  - Tian HB
FAU - Zhou, Yan-jun
AU  - Zhou YJ
FAU - Tong, Guang-zhi
AU  - Tong GZ
FAU - Li, Xi
AU  - Li X
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110602
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Gold Colloid)
RN  - 0 (Immunoconjugates)
RN  - 0 (Reagent Strips)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibodies, Viral/*immunology
MH  - China
MH  - Chromatography, Affinity/*methods
MH  - Gold Colloid
MH  - Immunoconjugates
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - *Reagent Strips/chemistry
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Time Factors
EDAT- 2011/06/15 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/06/14 06:00
PHST- 2011/02/16 00:00 [received]
PHST- 2011/05/05 00:00 [revised]
PHST- 2011/05/25 00:00 [accepted]
PHST- 2011/06/14 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0166-0934(11)00242-4 [pii]
AID - 10.1016/j.jviromet.2011.05.034 [doi]
PST - ppublish
SO  - J Virol Methods. 2011 Sep;176(1-2):46-52. doi: 10.1016/j.jviromet.2011.05.034. 
      Epub 2011 Jun 2.