PMID- 21664172
OWN - NLM
STAT- MEDLINE
DCOM- 20120126
LR  - 20211020
IS  - 1879-0402 (Electronic)
IS  - 1367-5931 (Print)
IS  - 1367-5931 (Linking)
VI  - 15
IP  - 5
DP  - 2011 Oct
TI  - Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.
PG  - 733-40
LID - 10.1016/j.cbpa.2011.05.016 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for 
      the detection of native and exogenous compounds in biological samples on a 
      cellular scale. Through the development of novel ion beams the amount of 
      molecular signal available from the sample surface has been increased. Through 
      the introduction of polyatomic ion beams, particularly C(60), ToF-SIMS can now be 
      used to monitor molecular signals as a function of depth as the sample is eroded 
      thus proving the ability to generate 3D molecular images. Here we describe how 
      this new capability has led to the development of novel instrumentation for 3D 
      molecular imaging while also highlighting the importance of sample preparation 
      and discuss the challenges that still need to be overcome to maximise the impact 
      of the technique.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Fletcher, John S
AU  - Fletcher JS
AD  - School of Chemical Engineering and Analytical Science, Manchester 
      Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK. 
      John.Fletcher@manchester.ac.uk
FAU - Vickerman, John C
AU  - Vickerman JC
FAU - Winograd, Nicholas
AU  - Winograd N
LA  - eng
GR  - R01 EB002016/EB/NIBIB NIH HHS/United States
GR  - R01 EB002016-17/EB/NIBIB NIH HHS/United States
GR  - EB-002016-17/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20110612
PL  - England
TA  - Curr Opin Chem Biol
JT  - Current opinion in chemical biology
JID - 9811312
RN  - 0 (Amino Acids)
RN  - 0 (Ions)
RN  - 0 (Phospholipids)
SB  - IM
MH  - Amino Acids/*analysis
MH  - Animals
MH  - Cell Membrane/*chemistry
MH  - HeLa Cells
MH  - Humans
MH  - Imaging, Three-Dimensional/instrumentation/*methods
MH  - Ions/chemistry
MH  - Molecular Imaging/instrumentation/*methods
MH  - Phospholipids/*analysis
MH  - Principal Component Analysis
MH  - Software
MH  - Spectrometry, Mass, Secondary Ion/instrumentation/*methods
MH  - Xenopus laevis
PMC - PMC3181283
MID - NIHMS299566
EDAT- 2011/06/15 06:00
MHDA- 2012/01/27 06:00
PMCR- 2012/10/01
CRDT- 2011/06/14 06:00
PHST- 2011/04/18 00:00 [received]
PHST- 2011/05/19 00:00 [revised]
PHST- 2011/05/20 00:00 [accepted]
PHST- 2011/06/14 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2012/01/27 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - S1367-5931(11)00085-8 [pii]
AID - 10.1016/j.cbpa.2011.05.016 [doi]
PST - ppublish
SO  - Curr Opin Chem Biol. 2011 Oct;15(5):733-40. doi: 10.1016/j.cbpa.2011.05.016. Epub 
      2011 Jun 12.