PMID- 21664481
OWN - NLM
STAT- MEDLINE
DCOM- 20111024
LR  - 20161125
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Linking)
VI  - 160
IP  - 1
DP  - 2011 Sep
TI  - Isolation and characterization of the CYP17A1 gene and its processed pseudogene 
      in Rana rugosa.
PG  - 54-61
AB  - CYP17A1 expression is up-regulated in the gonad in Rana (Glandirana) rugosa 
      tadpoles treated with androgens to induce female-to-male sex-reversal. In this 
      study, we isolated the CYP17A1 gene and its processed pseudogene from R. rugosa. 
      The former was found to consist of 8 exons, and the latter a single-exon gene, 
      designated CYP17A1P. The sequence of the promoter region of CYP17A1 differed from 
      that of CYP17A1P. We found several consensus binding-sites for candidate 
      transcription factors including androgen receptor (AR), Sox and FoxL2 in the 
      CYP17A1 promoter region, but an AR-binding site was absent from CYP17A1P. When AR 
      was over-expressed in Xenopus A6 cells, it did not increase CYP17A1 transcription 
      in luciferase assays. CYP17A1 was strongly expressed in indifferent male gonads 
      during sex determination and exclusively in testis, among eight adult tissues of 
      R. rugosa. By contrast, CYP17A1P was expressed at very low, and similar levels in 
      the adult tissues of both sexes. Fluorescent In-Situ Hybridization (FISH) 
      analysis showed that CYP17A1P is localized to chromosome 4, while CYP17A1 is on 
      chromosome 9. These results collectively suggest that CYP17A1, but not CYP17A1P 
      is involved in male sex-determination in R. rugosa, and that androgens may not 
      have a direct effect on the CYP17A1 transcription.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Suda, Mari
AU  - Suda M
AD  - Department of Biology, Waseda University, Wakamatsu-cho, Shinjuku-ku, Tokyo, 
      Japan.
FAU - Uno, Yoshinobu
AU  - Uno Y
FAU - Fujii, Jun
AU  - Fujii J
FAU - Matsuda, Yoichi
AU  - Matsuda Y
FAU - Nakamura, Masahisa
AU  - Nakamura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110602
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Transcription Factors)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Female
MH  - Gonads/enzymology/physiology
MH  - Male
MH  - Metamorphosis, Biological/physiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - *Pseudogenes
MH  - Ranidae/anatomy & histology/*genetics/*metabolism
MH  - Sex Determination Processes/physiology
MH  - Steroid 17-alpha-Hydroxylase/classification/*genetics/*isolation & 
      purification/metabolism
MH  - Tissue Distribution
MH  - Transcription Factors/genetics/metabolism
EDAT- 2011/06/15 06:00
MHDA- 2011/10/25 06:00
CRDT- 2011/06/14 06:00
PHST- 2011/03/03 00:00 [received]
PHST- 2011/05/18 00:00 [revised]
PHST- 2011/05/24 00:00 [accepted]
PHST- 2011/06/14 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2011/10/25 06:00 [medline]
AID - S1096-4959(11)00108-4 [pii]
AID - 10.1016/j.cbpb.2011.05.008 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2011 Sep;160(1):54-61. doi: 
      10.1016/j.cbpb.2011.05.008. Epub 2011 Jun 2.