PMID- 21664941
OWN - NLM
STAT- MEDLINE
DCOM- 20120111
LR  - 20110919
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 72
IP  - 10
DP  - 2011 Oct
TI  - Human leukocyte antigen-B (-Bw6/-Bw4 I80, T80) and human leukocyte antigen-C 
      (-C1/-C2) subgrouping using pyrosequence analysis.
PG  - 859-68
LID - 10.1016/j.humimm.2011.05.007 [doi]
AB  - Specific combinations of killer immunoglobulin-like receptors (KIR) and human 
      leukocyte antigen (HLA) class I molecules characterized by a particular residue 
      80 are significantly associated with outcomes in different pathologic conditions, 
      such as autoimmunity, pathogenic infection, cancer, and reproductive failure. 
      Thus, a simplified method for HLA typing used in association with the analysis of 
      KIR genotype (Kirotype) is of particular interest to extend the analysis of 
      larger series. Here, we describe a quick and inexpensive method that allows use 
      of pyrosequencing, a helpful subtyping of HLA class I molecules, into HLA-Bw6, 
      -Bw4 I(80) or -Bw4 T(80), HLA-C1, or -C2 groups and HLA-A allotypes sharing Bw4+ 
      epitope or the rare HLA-B allotypes displaying the C1 motif. In particular, this 
      analysis is focused on the amino acids around residue 80, known to be relevant in 
      defining the affinity of KIR/HLA interaction and in the functional effects. This 
      method was demonstrated to have good sensitivity, specificity, and 
      reproducibility of detection and it was validated using a panel of HLA-typed 
      International Histocompatibility Workshop (IHW) cell lines and clinical isolates. 
      Using an allele quantitative acquisition mode, the method permitted us to obtain 
      an accurate sequencing as required in heterozygous and/or homozygous sample 
      definition.
CI  - Copyright (c) 2011 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Ugolotti, Elisabetta
AU  - Ugolotti E
AD  - Molecular Medicine, Istituto Giannina Gaslini, Genoa, Italy.
FAU - Vanni, Irene
AU  - Vanni I
FAU - Raso, Alessandro
AU  - Raso A
FAU - Benzi, Fabio
AU  - Benzi F
FAU - Malnati, Mauro
AU  - Malnati M
FAU - Biassoni, Roberto
AU  - Biassoni R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110524
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Epitopes)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (Receptors, KIR3DL1)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Autoimmunity/*genetics
MH  - Cell Line
MH  - Epitopes/*analysis/genetics/immunology
MH  - Genotype
MH  - HLA-B Antigens/*analysis/genetics/immunology
MH  - HLA-C Antigens/*analysis/genetics/immunology
MH  - Heterozygote
MH  - Histocompatibility Testing/*methods
MH  - Homozygote
MH  - Humans
MH  - Leukocytes/*chemistry/cytology/immunology
MH  - Molecular Sequence Data
MH  - Molecular Typing/*methods
MH  - Receptors, KIR3DL1/*analysis/genetics/immunology
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA/*methods
EDAT- 2011/06/15 06:00
MHDA- 2012/01/12 06:00
CRDT- 2011/06/14 06:00
PHST- 2011/01/21 00:00 [received]
PHST- 2011/05/03 00:00 [revised]
PHST- 2011/05/13 00:00 [accepted]
PHST- 2011/06/14 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2012/01/12 06:00 [medline]
AID - S0198-8859(11)00114-5 [pii]
AID - 10.1016/j.humimm.2011.05.007 [doi]
PST - ppublish
SO  - Hum Immunol. 2011 Oct;72(10):859-68. doi: 10.1016/j.humimm.2011.05.007. Epub 2011 
      May 24.