PMID- 21668478
OWN - NLM
STAT- MEDLINE
DCOM- 20111129
LR  - 20191210
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 59
IP  - 1
DP  - 2011 Jul
TI  - Prognostic value of partial genetic instability in neuroblastoma with </=50% 
      neuroblastic cell content.
PG  - 22-30
LID - 10.1111/j.1365-2559.2011.03899.x [doi]
AB  - AIMS: Better understanding of neuroblastoma genetics will improve with 
      genome-wide techniques. However, performing these analyses in samples with <60% 
      neuroblast cells is not adequate. We evaluated the utility of fluorescence in 
      situ hybridization (FISH) on tissue microarrays (TMA) in detecting partial 
      genetic instability (PGI), focusing on samples with </=50% neuroblast cells. 
      METHODS AND RESULTS: Alterations of 11q and 17q were detected by FISH on 369 
      neuroblastoma samples in TMA. Status of the MYCN gene and 1p36 region has been 
      established previously by FISH diagnosis. Partial genetic instability (PGI) was 
      defined as the ratio between segmental genetic alterations detected and number of 
      genetic markers diagnosed in each tumour. Of primary tumours, 14.6% harboured 11q 
      deletions, whereas 42.6% showed 17q gain. PGI was established in 260 primary 
      tumours, 67 of which contained </=50% neuroblasts. Outcomes were statistically 
      worse for patients whose tumours presented high PGI (P < 0.0001). Multivariate 
      analysis revealed moderate and high PGI as prognostic factors. CONCLUSIONS: In 
      the cohort examined in this study, univariate and multivariate analysis confirmed 
      the effect of PGI in patient outcome. PGI established by FISH on TMA is a useful 
      method to identify high-risk patients even if tumours have a cell content of </=50% 
      neuroblast cells.
CI  - (c) 2011 Blackwell Publishing Limited.
FAU - Piqueras, Marta
AU  - Piqueras M
AD  - Department of Pathology, Medical School, University of Valencia, Spain.
FAU - Navarro, Samuel
AU  - Navarro S
FAU - Canete, Adela
AU  - Canete A
FAU - Castel, Victoria
AU  - Castel V
FAU - Noguera, Rosa
AU  - Noguera R
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110613
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Genetic Markers)
RN  - 0 (MYCN protein, human)
RN  - 0 (N-Myc Proto-Oncogene Protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins)
SB  - IM
MH  - Adolescent
MH  - Cell Count
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Genetic Markers
MH  - *Genomic Instability
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Kaplan-Meier Estimate
MH  - Multivariate Analysis
MH  - N-Myc Proto-Oncogene Protein
MH  - Neuroblastoma/*genetics/*pathology
MH  - Nuclear Proteins/genetics
MH  - Oncogene Proteins/genetics
MH  - Prognosis
MH  - Tissue Array Analysis
MH  - Young Adult
EDAT- 2011/06/15 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/06/15 06:00
PHST- 2011/06/15 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1111/j.1365-2559.2011.03899.x [doi]
PST - ppublish
SO  - Histopathology. 2011 Jul;59(1):22-30. doi: 10.1111/j.1365-2559.2011.03899.x. Epub 
      2011 Jun 13.