PMID- 21668953
OWN - NLM
STAT- MEDLINE
DCOM- 20110916
LR  - 20211020
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 11
DP  - 2011 Jun 13
TI  - A short-term in vivo model for giant cell tumor of bone.
PG  - 241
LID - 10.1186/1471-2407-11-241 [doi]
AB  - BACKGROUND: Because of the lack of suitable in vivo models of giant cell tumor of 
      bone (GCT), little is known about its underlying fundamental pro-tumoral events, 
      such as tumor growth, invasion, angiogenesis and metastasis. There is no existing 
      cell line that contains all the cell and tissue tumor components of GCT and thus 
      in vitro testing of anti-tumor agents on GCT is not possible. In this study we 
      have characterized a new method of growing a GCT tumor on a chick 
      chorio-allantoic membrane (CAM) for this purpose. METHODS: Fresh tumor tissue was 
      obtained from 10 patients and homogenized. The suspension was grafted onto the 
      CAM at day 10 of development. The growth process was monitored by daily 
      observation and photo documentation using in vivo biomicroscopy. After 6 days, 
      samples were fixed and further analyzed using standard histology (hematoxylin and 
      eosin stains), Ki67 staining and fluorescence in situ hybridization (FISH). 
      RESULTS: The suspension of all 10 patients formed solid tumors when grafted on 
      the CAM. In vivo microscopy and standard histology revealed a rich 
      vascularization of the tumors. The tumors were composed of the typical components 
      of GCT, including (CD51+/CD68+) multinucleated giant cells which were generally 
      less numerous and contained fewer nuclei than in the original tumors. Ki67 
      staining revealed a very low proliferation rate. The FISH demonstrated that the 
      tumors were composed of human cells interspersed with chick-derived capillaries. 
      CONCLUSIONS: A reliable protocol for grafting of human GCT onto the chick 
      chorio-allantoic membrane is established. This is the first in vivo model for 
      giant cell tumors of bone which opens new perspectives to study this disease and 
      to test new therapeutical agents.
FAU - Balke, Maurice
AU  - Balke M
AD  - Department of Trauma and Orthopedic Surgery, University of Witten-Herdecke, 
      Cologne-Merheim Medical Center, Ostmerheimer Str, Cologne, Germany. 
      maurice.balke@gmail.com
FAU - Neumann, Anna
AU  - Neumann A
FAU - Szuhai, Karoly
AU  - Szuhai K
FAU - Agelopoulos, Konstantin
AU  - Agelopoulos K
FAU - August, Christian
AU  - August C
FAU - Gosheger, Georg
AU  - Gosheger G
FAU - Hogendoorn, Pancras Cw
AU  - Hogendoorn PC
FAU - Athanasou, Nick
AU  - Athanasou N
FAU - Buerger, Horst
AU  - Buerger H
FAU - Hagedorn, Martin
AU  - Hagedorn M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110613
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Bone Neoplasms/diagnosis/*pathology
MH  - Chick Embryo
MH  - *Disease Models, Animal
MH  - Female
MH  - Giant Cell Tumor of Bone/diagnosis/*metabolism/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - Osteoclasts/cytology
MH  - *Xenograft Model Antitumor Assays
MH  - Young Adult
PMC - PMC3125284
EDAT- 2011/06/15 06:00
MHDA- 2011/09/17 06:00
PMCR- 2011/06/13
CRDT- 2011/06/15 06:00
PHST- 2010/12/16 00:00 [received]
PHST- 2011/06/13 00:00 [accepted]
PHST- 2011/06/15 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2011/09/17 06:00 [medline]
PHST- 2011/06/13 00:00 [pmc-release]
AID - 1471-2407-11-241 [pii]
AID - 10.1186/1471-2407-11-241 [doi]
PST - epublish
SO  - BMC Cancer. 2011 Jun 13;11:241. doi: 10.1186/1471-2407-11-241.