PMID- 2166908
OWN - NLM
STAT- MEDLINE
DCOM- 19900917
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 231
IP  - 2
DP  - 1990 Aug
TI  - The induction of chromosomal aberrations and SCEs by visible light in combination 
      with dyes. II. Cell cycle dependence, and the effect of hydroxyl radical 
      scavengers during light exposure in cultures of Chinese hamster ovary cells 
      sensitized with acridine orange.
PG  - 233-42
AB  - Chinese hamster ovary (CHO) cells were synchronized by mitotic shake-off, treated 
      with the fluorochrome acridine orange (AO; 0.5 micrograms/ml), washed free of 
      excess dye and subsequently exposed to visible light (2 X 40 W/8 Wm-2). The light 
      exposure was performed on cells in the G1, G1/S, S or G2 phase of the cell cycle. 
      AO + light induced high frequencies of aberration in the S phase and even higher 
      in the G1 phase. The aberrations observed were all of the chromatid type. The 
      chromosome-type aberrations (dicentrics, rings) obtained when cells in the G1 
      phase were exposed to X-rays were not found after corresponding treatments with 
      AO + light. With the exception of an increased frequency of gaps, no chromosomal 
      aberrations were induced in G2-phase cells. Sister-chromatid exchanges were 
      efficiently produced by the photodynamic system in the G1, G1/S and S phase of 
      the cell cycle. In other experiments, AO-treated unsynchronized CHO cells were 
      exposed to light in the presence of the hydroxyl radical scavengers mannitol (100 
      mM) and 5-dimethyl thiourea (100 mM). In parallel experiments these scavengers 
      were found to reduce markedly the chromosome breaking effects by X-rays but had 
      no influence on the photodynamic induction of chromosomal alterations. The 
      results presented show that the visible light-induced chromosomal alterations in 
      CHO cells sensitized with the fluorochrome AO are obtained by an S-dependent 
      mechanism. Furthermore, the results indicate that the hydroxyl free radical does 
      not play a major role in the production of chromosomal alterations by AO + light.
FAU - Uggla, A H
AU  - Uggla AH
AD  - Department of Genetics, University of Uppsala, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Free Radicals)
RN  - 0 (Hydroxides)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 3OWL53L36A (Mannitol)
RN  - 8P30PMD17W (1,3-dimethylthiourea)
RN  - F30N4O6XVV (Acridine Orange)
RN  - GYV9AM2QAG (Thiourea)
SB  - IM
MH  - Acridine Orange/*toxicity
MH  - Animals
MH  - Cell Cycle
MH  - Cell Line
MH  - *Chromosome Aberrations
MH  - Chromosomes/*drug effects/radiation effects
MH  - Cricetinae
MH  - Dose-Response Relationship, Radiation
MH  - Free Radicals
MH  - Hydroxides
MH  - Hydroxyl Radical
MH  - Light
MH  - Mannitol/pharmacology
MH  - *Sister Chromatid Exchange
MH  - Thiourea/analogs & derivatives/pharmacology
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 0027-5107(90)90029-4 [pii]
AID - 10.1016/0027-5107(90)90029-4 [doi]
PST - ppublish
SO  - Mutat Res. 1990 Aug;231(2):233-42. doi: 10.1016/0027-5107(90)90029-4.