PMID- 2166957
OWN - NLM
STAT- MEDLINE
DCOM- 19900920
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 87
IP  - 16
DP  - 1990 Aug
TI  - 1 alpha,25-dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II 
      in nonerythroid avian bone marrow cells.
PG  - 6470-4
AB  - 1 alpha,25-Dihydroxyvitamin D3 [1,25(OH)2D3], the active metabolite of the 
      steroid hormone vitamin D, is a potent regulator of macrophage and osteoclast 
      differentiation. The mature osteoclast, unlike the circulating monocyte or the 
      tissue macrophage, expresses high levels of carbonic anhydrase II (CAII). This 
      enzyme generates protons and bicarbonate from water and carbon dioxide and is 
      involved in bone resorption and acid-base regulation. To test whether 1,25(OH)2D3 
      could induce the differentiation of myelomonocytic precursors toward osteoclasts 
      rather than macrophages, we analyzed its effects on the expression of CAII in 
      bone marrow cultures containing precursors common to both cell types. The 
      expression of CAII was markedly increased by 1,25(OH)2D3 in a dose- and 
      time-dependent manner. In bone marrow, this increase occurred at the mRNA and 
      protein levels and was detectable as early as 24 hr after stimulation. 
      1,25(OH)2D3 was also found to induce CAII expression in a transformed 
      myelomonocytic avian cell line. These results suggest that 1,25(OH)2D3 regulates 
      the level at which myelomonocytic precursors express CAII, an enzyme that is 
      involved in the function of the mature osteoclast.
FAU - Billecocq, A
AU  - Billecocq A
AD  - Department of Cell Biology, Yale University School of Medicine, New Haven, CT 
      06510.
FAU - Emanuel, J R
AU  - Emanuel JR
FAU - Levenson, R
AU  - Levenson R
FAU - Baron, R
AU  - Baron R
LA  - eng
GR  - DE-04724/DE/NIDCR NIH HHS/United States
GR  - HL-39263/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Probes)
RN  - 0 (Isoenzymes)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Avian Leukosis Virus/*genetics
MH  - Avian Myeloblastosis Virus/*genetics
MH  - Blotting, Western
MH  - Bone Marrow/drug effects/*enzymology
MH  - Calcitriol/*pharmacology
MH  - Carbonic Anhydrases/*biosynthesis/genetics/isolation & purification
MH  - Cell Line
MH  - Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - Chickens
MH  - DNA Probes
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Isoenzymes/*biosynthesis/genetics/isolation & purification
MH  - Kinetics
PMC - PMC54556
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
PMCR- 1991/02/01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
PHST- 1991/02/01 00:00 [pmc-release]
AID - 10.1073/pnas.87.16.6470 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1990 Aug;87(16):6470-4. doi: 10.1073/pnas.87.16.6470.