PMID- 2167186
OWN - NLM
STAT- MEDLINE
DCOM- 19900927
LR  - 20190510
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 81
IP  - 2
DP  - 1990 Aug
TI  - Stimulation of neutrophil elastase and myeloperoxidase release by IgG fragments.
PG  - 352-6
AB  - Human leucocyte elastase (HLE) cleaves IgG into Fab and Fc fragments. The Fc 
      fragment bears an elastase-specific antigen and has previously been reported to 
      be found in synovial fluid during rheumatoid arthritis. In addition, biological 
      activity of elastase-specific Fc fragments has been described in modulating 
      granulocyte oxidative metabolism. To investigate further regulatory effects of 
      the elastase-induced IgG cleavage products, we tested the elastase and 
      myeloperoxidase release of granulocytes. IgG fragments induce no enzyme release 
      of unstimulated neutrophils. But elastase and myeloperoxidase release of 
      cytochalasin b/FMLP-treated neutrophils is stimulated in a dose-dependent manner 
      by the Fab fragments. The extent of stimulation depends on stimulus concentration 
      and is at its maximum for low (e.g. 2.5 x 10(-8) M) FMLP concentration. Ten 
      nanomoles Fab/4 x 10(6) PMN augment elastase release to 206% and myeloperoxidase 
      release to 155% after pre-stimulation with 2.5 x 10(-8) M FMLP. Fc fragments 
      stimulate elastase release to 162% but no MPO release. Untreated IgG1 and analog 
      Fab and Fc fragments produced by papain cleavage react similarly. 
      Elastase-generated IgG fragments may therefore up-regulate their concentration by 
      simulating elastase release. The concomitantly stimulated release of 
      myeloperoxidase may influence bactericidal activity and termination of oxidative 
      burst.
FAU - Eckle, I
AU  - Eckle I
AD  - Centre for Internal Medicine, Philipps University, Marburg, West Germany.
FAU - Kolb, G
AU  - Kolb G
FAU - Heiser, C
AU  - Heiser C
FAU - Havemann, K
AU  - Havemann K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
SB  - IM
MH  - Cytochalasin B/pharmacology
MH  - Humans
MH  - Immunoglobulin Fab Fragments/physiology
MH  - Immunoglobulin Fc Fragments/physiology
MH  - Immunoglobulin G/metabolism/*physiology
MH  - In Vitro Techniques
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*enzymology
MH  - Pancreatic Elastase/*metabolism
MH  - Peroxidase/*metabolism
MH  - Serum Albumin, Bovine/pharmacology
PMC - PMC1535046
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
PMCR- 1991/08/01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
PHST- 1991/08/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.1990.tb03344.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1990 Aug;81(2):352-6. doi: 10.1111/j.1365-2249.1990.tb03344.x.