PMID- 21672382
OWN - NLM
STAT- MEDLINE
DCOM- 20120130
LR  - 20131121
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 23
IP  - 6
DP  - 2011 Jun
TI  - [The role of Leptin on neuron apoptosis in mice with cerebral 
      ischemia/reperfusion injury].
PG  - 345-8
AB  - OBJECTIVE: To study the effect of Leptin on neuron apoptosis in mice with 
      cerebral ischemia injury and its mechanism. METHODS: Seventy-five mice were 
      randomly divided into three groups. Focal cerebral ischemia/reperfusion injury 
      model in mice was reproduced by middle cerebral artery occlusion for 2 hours 
      followed by reperfusion. In Leptin intervention group mice were given Leptin 1 
      mug/g during cerebral ischemia by intraperitoneal injection. Mice in the model 
      group were given equal amount of phosphate buffer saline. After reperfusion for 
      24 hours, the neuron apoptosis was detected by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. The mRNA and 
      protein expression of apoptosis relative gene caspase-3 and bcl-2 were determined 
      by reverse transcription-polymerase chain reaction (RT-PCR) and immuno 
      histochemistry. RESULTS: Most of neuron necrosis was observed in cerebral 
      ischemia center in model group. Compared with sham-operation group, neuron 
      apoptosis rate, mRNA and protein expression of caspase-3 and bcl-2 in model group 
      increased significantly [apoptosis rate: (68.65 +/- 0.79)% vs. (4.40 +/- 0.00)%, 
      caspase-3 mRNA: 2.563 +/- 0.250 vs. 0.153 +/- 0.020, bcl-2 mRNA: 0.337 +/- 0.100 vs. 
      0.125 +/- 0.030, caspase-3 protein (absorbance value, A value): 0.57 +/- 0.05 vs. 
      0.37 +/- 0.03, bcl-2 protein (A value): 0.51 +/- 0.04 vs. 0.35 +/- 0.01, all P<0.01]. 
      The apoptosis rate of penumbra neurons was reduced in Leptin intervention group 
      significantly compared with model group [(42.30 +/- 8.45)% vs. (68.65 +/- 0.79)%, 
      P<0.01]. Compared with model group, the mRNA and protein expression of caspase-3 
      in Leptin intervention group were reduced significantly [caspase-3 mRNA: 2.267 +/- 
      0.040 vs. 2.563 +/- 0.250, caspase-3 protein (A value): 0.45 +/- 0.04 vs. 0.57 +/- 
      0.05, P>0.05 and P<0.01], and the mRNA and protein expression of bcl-2 in Leptin 
      intervention group upregulated significantly [bcl-2 mRNA: 0.662 +/- 0.040 vs. 0.337 
      +/- 0.100, bcl-2 protein (A value): 0.76 +/- 0.09 vs. 0.51 +/- 0.04, both P<0.01]. 
      CONCLUSION: Leptin could reduce apoptosis of neurons through down-regulation of 
      the expression of caspase-3 and up-regulation of the expression of bcl-2. The 
      results suggest that Leptin plays a neuroprotective role in cerebral ischemia 
      injury.
FAU - Yan, Guang-tao
AU  - Yan GT
AD  - Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of 
      PLA, Beijing 100853, China. yan301@263.net
FAU - Si, Yi-ling
AU  - Si YL
FAU - Zhang, Jin-ying
AU  - Zhang JY
FAU - Deng, Zi-hui
AU  - Deng ZH
FAU - Xue, Hui
AU  - Xue H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Leptin)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 114100-40-2 (Bcl2 protein, mouse)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Brain Ischemia/metabolism/*pathology
MH  - Caspase 3/metabolism
MH  - Leptin/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Neurons/cytology/*drug effects/metabolism
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - Reperfusion Injury/metabolism/*pathology
EDAT- 2011/06/16 06:00
MHDA- 2012/01/31 06:00
CRDT- 2011/06/16 06:00
PHST- 2011/06/16 06:00 [entrez]
PHST- 2011/06/16 06:00 [pubmed]
PHST- 2012/01/31 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2011 Jun;23(6):345-8.