PMID- 21674707
OWN - NLM
STAT- MEDLINE
DCOM- 20111028
LR  - 20211020
IS  - 1536-4844 (Electronic)
IS  - 1078-0998 (Print)
IS  - 1078-0998 (Linking)
VI  - 17
IP  - 7
DP  - 2011 Jul
TI  - Maternal obesity induces sustained inflammation in both fetal and offspring large 
      intestine of sheep.
PG  - 1513-22
LID - 10.1002/ibd.21539 [doi]
AB  - BACKGROUND: Both maternal obesity and inflammatory bowel diseases (IBDs) are 
      increasing. It was hypothesized that maternal obesity induces an inflammatory 
      response in the fetal large intestine, predisposing offspring to IBDs. METHODS: 
      Nonpregnant ewes were assigned to a control (Con, 100% of National Research 
      Council [NRC] recommendations) or obesogenic (OB, 150% of NRC) diet from 60 days 
      before conception. The large intestine was sampled from fetuses at 135 days (term 
      150 days) after conception and from offspring lambs at 22.5 +/- 0.5 months of age. 
      RESULTS: Maternal obesity enhanced mRNA expression tumor necrosis factor (TNF)alpha, 
      interleukin (IL)1alpha, IL1beta, IL6, IL8, and monocyte/macrophage chemotactic protein-1 
      (MCP1), as well as macrophage markers, CD11b, CD14, and CD68 in fetal gut. mRNA 
      expression of Toll-like receptor (TLR) 2 and TLR4 was increased in OB versus Con 
      fetuses; correspondingly, inflammatory NF-kappaB and JNK signaling pathways were also 
      upregulated. Both mRNA expression and protein content of transforming growth 
      factor (TGF) beta was increased. The IL-17A mRNA expression and protein content was 
      higher in OB compared to Con samples, which was associated with fibrosis in the 
      large intestine of OB fetuses. Similar inflammatory responses and enhanced 
      fibrosis were detected in OB compared to Con offspring. CONCLUSIONS: Maternal 
      obesity induced inflammation and enhanced expression of proinflammatory cytokines 
      in fetal and offspring large intestine, which correlated with increased TGFbeta and 
      IL17 expression. These data show that maternal obesity may predispose offspring 
      gut to IBDs.
CI  - Copyright (c) 2010 Crohn's & Colitis Foundation of America, Inc.
FAU - Yan, Xu
AU  - Yan X
AD  - Center for the Study of Fetal Programming, Department of Animal Science, 
      University of Wyoming, Laramie, Wyoming, USA.
FAU - Huang, Yan
AU  - Huang Y
FAU - Wang, Hui
AU  - Wang H
FAU - Du, Min
AU  - Du M
FAU - Hess, Bret W
AU  - Hess BW
FAU - Ford, Stephen P
AU  - Ford SP
FAU - Nathanielsz, Peter W
AU  - Nathanielsz PW
FAU - Zhu, Mei-Jun
AU  - Zhu MJ
LA  - eng
GR  - P20 RR016474/RR/NCRR NIH HHS/United States
GR  - P20 RR016474-10/RR/NCRR NIH HHS/United States
GR  - P20RR016474/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20101128
PL  - England
TA  - Inflamm Bowel Dis
JT  - Inflammatory bowel diseases
JID - 9508162
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-17)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*immunology
MH  - Blotting, Western
MH  - Cytokines/genetics/metabolism
MH  - Female
MH  - Fetus/immunology/*pathology
MH  - Fibrosis/*etiology/pathology
MH  - Inflammation/*etiology/pathology
MH  - Interleukin-17/genetics/metabolism
MH  - Intestine, Large/*immunology/metabolism/pathology
MH  - Maternal Nutritional Physiological Phenomena
MH  - NF-kappa B/genetics/metabolism
MH  - Obesity/*complications
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sheep
MH  - Transforming Growth Factor beta/genetics/metabolism
PMC - PMC3116110
MID - NIHMS240498
EDAT- 2011/06/16 06:00
MHDA- 2011/10/29 06:00
PMCR- 2012/07/01
CRDT- 2011/06/16 06:00
PHST- 2010/09/17 00:00 [received]
PHST- 2010/09/23 00:00 [accepted]
PHST- 2011/06/16 06:00 [entrez]
PHST- 2011/06/16 06:00 [pubmed]
PHST- 2011/10/29 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - 10.1002/ibd.21539 [doi]
PST - ppublish
SO  - Inflamm Bowel Dis. 2011 Jul;17(7):1513-22. doi: 10.1002/ibd.21539. Epub 2010 Nov 
      28.