PMID- 21677395
OWN - NLM
STAT- MEDLINE
DCOM- 20110822
LR  - 20211020
IS  - 0973-7731 (Electronic)
IS  - 0022-1333 (Linking)
VI  - 90
IP  - 1
DP  - 2011 Apr
TI  - Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH 
      and banding pattern comparisons.
PG  - 111-7
AB  - The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), 
      considered the most ancestral one, shows the greatest amount of heterochromatin 
      described among Platyrrhini genera. Banding techniques and restriction enzyme 
      digestion have previously revealed great variability of quantity and composition 
      of heterochromatin in this genus. In this context, we use fluorescence in situ 
      hybridization (FISH) to analyse this genomic region and discuss its possible role 
      in the diversification of Cebus.We used a heterochromatin probe for chromosome 11 
      of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. 
      Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae 
      and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were 
      Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. 
      nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 
      6 to 22 positive signals located in interstitial and telomeric positions along 
      the different species. No hybridization signal was observed among the remaining 
      Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin 
      composed of a complex system of repetitive sequences.
FAU - Nieves, Mariela
AU  - Nieves M
AD  - Grupo de Investigacion en Biologia Evolutiva, Laboratorio 46, 4 degrees  piso, Facultad 
      de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II - 
      Ciudad Universitaria, Intendente Guiraldes 2160 - C1428EGA, Ciudad Autonoma de 
      Buenos Aires, Argentina. mnieves@ege.fcen.uba.ar
FAU - De Oliveira, Edivaldo H C
AU  - De Oliveira EH
FAU - Amaral, Paulo J S
AU  - Amaral PJ
FAU - Nagamachi, Cleusa Y
AU  - Nagamachi CY
FAU - Pieczarka, Julio C
AU  - Pieczarka JC
FAU - Muhlmann, Maria C
AU  - Muhlmann MC
FAU - Mudry, Marta D
AU  - Mudry MD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - J Genet
JT  - Journal of genetics
JID - 2985113R
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Cebus/*genetics
MH  - Chromosome Banding
MH  - Chromosomes/*genetics
MH  - Heterochromatin/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Microdissection
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2011/06/17 06:00
MHDA- 2011/08/23 06:00
CRDT- 2011/06/17 06:00
PHST- 2011/06/17 06:00 [entrez]
PHST- 2011/06/17 06:00 [pubmed]
PHST- 2011/08/23 06:00 [medline]
AID - 10.1007/s12041-011-0047-0 [doi]
PST - ppublish
SO  - J Genet. 2011 Apr;90(1):111-7. doi: 10.1007/s12041-011-0047-0.