PMID- 21677511
OWN - NLM
STAT- MEDLINE
DCOM- 20111222
LR  - 20211020
IS  - 1933-6926 (Electronic)
IS  - 1933-6918 (Print)
IS  - 1933-6918 (Linking)
VI  - 5
IP  - 4
DP  - 2011 Jul-Aug
TI  - Tuba and N-WASP function cooperatively to position the central lumen during 
      epithelial cyst morphogenesis.
PG  - 344-50
AB  - The process of epithelial lumenogenesis requires coordination of a network of 
      signaling machinery communicated to each cell through subsequent cell divisions. 
      Formation of a single hollow lumen has previously been shown to require Tuba, a 
      Cdc42 GEF, for Cdc42 activation and correct spindle orientation. Using a Caco-2 
      model of lumenogenesis, we show that knockdown (KD) of the actin regulator 
      N-WASP, causes a multilumen phenotype similar to Tuba KD. Defects in 
      lumenogenesis in Tuba KD and N-WASP KD cells are observed at the two cell stage 
      with inappropriate marking of the pre-apical patch (PAP) - the precursor to lumen 
      formation. Strikingly, both Tuba and N-WASP depend on each other for localization 
      to the PAP. We conclude that N-WASP functions cooperatively with Tuba to 
      facilitate lumenogenesis and this requires the polyproline region of N-WASP.
FAU - Kovacs, Eva M
AU  - Kovacs EM
AD  - Division of Molecular Cell Biology, Institute for Molecular Bioscience, The 
      University of Queensland-St. Lucia, QLD, Australia.
FAU - Verma, Suzie
AU  - Verma S
FAU - Thomas, Steven G
AU  - Thomas SG
FAU - Yap, Alpha S
AU  - Yap AS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110701
PL  - United States
TA  - Cell Adh Migr
JT  - Cell adhesion & migration
JID - 101469464
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNMBP protein, human)
RN  - 0 (Peptides)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (WASL protein, human)
RN  - 0 (Wiskott-Aldrich Syndrome Protein, Neuronal)
RN  - 25191-13-3 (polyproline)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Antigens, CD
MH  - Caco-2 Cells
MH  - Cadherins/metabolism
MH  - Cell Division
MH  - Cell Polarity
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/*metabolism
MH  - Epithelial Cells/cytology/*metabolism
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Lentivirus/genetics
MH  - *Morphogenesis
MH  - Peptides/metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Signal Transduction
MH  - Spindle Apparatus/metabolism
MH  - Transfection
MH  - Wiskott-Aldrich Syndrome Protein, Neuronal/*metabolism
MH  - cdc42 GTP-Binding Protein/metabolism
PMC - PMC3210302
EDAT- 2011/06/17 06:00
MHDA- 2011/12/23 06:00
PMCR- 2012/07/01
CRDT- 2011/06/17 06:00
PHST- 2011/06/17 06:00 [entrez]
PHST- 2011/06/17 06:00 [pubmed]
PHST- 2011/12/23 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - 16717 [pii]
AID - 1933-6918-5-4-9 [pii]
AID - 10.4161/cam.5.4.16717 [doi]
PST - ppublish
SO  - Cell Adh Migr. 2011 Jul-Aug;5(4):344-50. doi: 10.4161/cam.5.4.16717. Epub 2011 
      Jul 1.