PMID- 21679749
OWN - NLM
STAT- MEDLINE
DCOM- 20111214
LR  - 20110718
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 500
IP  - 1
DP  - 2011 Aug 1
TI  - Accessory olfactory bulb neurons are required for maintenance but not induction 
      of V2R vomeronasal receptor gene expression in vitro.
PG  - 6-9
LID - 10.1016/j.neulet.2011.05.232 [doi]
AB  - Many mammals detect pheromones by a sensory organ, the vomeronasal organ (VNO). 
      In a previous study using immunoblot and immunocytochemical analyses, we reported 
      that cocultures of VNOs with accessory olfactory bulb (AOB) neurons resulted in 
      the maturation of vomeronasal sensory neurons (VSNs) and a greater expression of 
      V2R family vomeronasal receptors than cultures with VNO alone. To further 
      characterize the V2R expression, we here investigated the time course of the 
      expression of V2R mRNA in the presence or absence of AOB neurons using RT-PCR 
      analysis. The expression of V2R mRNA was already detectable not only in the VNO 
      cocultured with AOB neurons for 3 days in coculture but also in the VNO cultured 
      alone for the same number of days. However, the expression of V2R mRNA in the VNO 
      cultured alone was remarkably decreased during the additional culture period, 
      although that in the cocultured VNO showed sustained expression. Moreover, the 
      application of 2 muM TTX to the cocultured VNO resulted in a marked decrease in 
      the V2R mRNA expression to a level equal to that in the VNO cultured alone for 14 
      days in coculture. Our previous working hypothesis was that the expression of 
      V2Rs in VSNs was induced by interacting with AOB neurons. However, the present 
      results suggest that the receptor expression in VSNs is independent of the 
      interaction with AOB neurons in the early developmental stage, but is maintained 
      by the active interaction with AOB neurons.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Muramoto, Kazuyo
AU  - Muramoto K
AD  - Division of Physiology, Department of Human Development and Fostering, Meikai 
      University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan. 
      tkazuyo@dent.maikai.ac.jp
FAU - Hagino-Yamagishi, Kimiko
AU  - Hagino-Yamagishi K
FAU - Tonosaki, Keiichi
AU  - Tonosaki K
FAU - Kaba, Hideto
AU  - Kaba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110606
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Vasopressin)
SB  - IM
MH  - Animals
MH  - Coculture Techniques
MH  - Embryo, Mammalian/cytology
MH  - Neurons/cytology/*metabolism
MH  - Olfactory Bulb/cytology/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Vasopressin/genetics/*metabolism
MH  - Time Factors
MH  - Vomeronasal Organ/cytology/metabolism
EDAT- 2011/06/18 06:00
MHDA- 2011/12/15 06:00
CRDT- 2011/06/18 06:00
PHST- 2011/03/31 00:00 [received]
PHST- 2011/05/17 00:00 [revised]
PHST- 2011/05/27 00:00 [accepted]
PHST- 2011/06/18 06:00 [entrez]
PHST- 2011/06/18 06:00 [pubmed]
PHST- 2011/12/15 06:00 [medline]
AID - S0304-3940(11)00868-8 [pii]
AID - 10.1016/j.neulet.2011.05.232 [doi]
PST - ppublish
SO  - Neurosci Lett. 2011 Aug 1;500(1):6-9. doi: 10.1016/j.neulet.2011.05.232. Epub 
      2011 Jun 6.