PMID- 21681438
OWN - NLM
STAT- MEDLINE
DCOM- 20120724
LR  - 20220321
IS  - 1436-6215 (Electronic)
IS  - 1436-6207 (Linking)
VI  - 51
IP  - 3
DP  - 2012 Apr
TI  - Triacylglycerol-rich lipoproteins derived from healthy donors fed different olive 
      oils modulate cytokine secretion and cyclooxygenase-2 expression in macrophages: 
      the potential role of oleanolic acid.
PG  - 301-9
LID - 10.1007/s00394-011-0215-2 [doi]
AB  - PURPOSE: Current evidence suggests that consumption of virgin olive oil (VOO) 
      helps to protect against the development of atherosclerosis and that minor 
      components such as oleanolic acid contribute to this effect. In this study, the 
      effects of triacylglycerol-rich lipoproteins (TRLs) derived from olive oil on 
      inflammatory processes in macrophages and how they are modulated by oleanolic 
      acid was investigated. METHODS: TRLs isolated from healthy volunteers 2 and 4 h 
      after a test meal containing VOO, pomace olive oil (POO) (the second pressing of 
      olive oil, enriched in minor components) or POO enriched with oleanolic acid 
      (OPOO) were incubated with macrophages derived from the human monocyte cell line, 
      THP-1. RESULTS: All types of TRLs caused a decrease of about 50% in the secretion 
      of monocyte chemoattractant protein-1 (MCP-1) by the cells. Interleukin (IL)-6 
      secretion was also significantly decreased by 2 and 4 h VOO TRLs and by 4 h OPOO 
      TRLs. In contrast, increased IL-1beta secretion was observed with all 2 h TRL types, 
      and increased tumour necrosis factor-alpha (TNF-alpha) production with 2 h VOO and POO, 
      but not OPOO, TRLs. TRLs isolated after 4 h, however, had no significant effects 
      on TNF-alpha secretion and increased IL-1beta secretion only when they were derived from 
      VOO. Cyclooxygenase-2 (COX-2) mRNA expression was strongly down-regulated by all 
      types of TRLs, but protein expression was significantly depressed only by 4 h 
      OPOO TRLs. CONCLUSION: These findings demonstrate that TRLs derived from olive 
      oil influence inflammatory processes in macrophages and suggest that oleanolic 
      acid may have beneficial effects.
FAU - Graham, V S
AU  - Graham VS
AD  - Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal 
      College St, London, NW1 0TU, UK.
FAU - Lawson, C
AU  - Lawson C
FAU - Wheeler-Jones, C P D
AU  - Wheeler-Jones CP
FAU - Perona, J S
AU  - Perona JS
FAU - Ruiz-Gutierrez, V
AU  - Ruiz-Gutierrez V
FAU - Botham, K M
AU  - Botham KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110617
PL  - Germany
TA  - Eur J Nutr
JT  - European journal of nutrition
JID - 100888704
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipoproteins)
RN  - 0 (Olive Oil)
RN  - 0 (Plant Oils)
RN  - 0 (RNA, Messenger)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 6SMK8R7TGJ (Oleanolic Acid)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
SB  - IM
MH  - Adult
MH  - Cell Line
MH  - Chemokine CCL2/drug effects/metabolism
MH  - Cyclooxygenase 2/genetics/*metabolism
MH  - Down-Regulation
MH  - Humans
MH  - Interleukin-1beta/drug effects/*metabolism
MH  - Interleukin-6/metabolism
MH  - Lipoproteins/*metabolism
MH  - Macrophages/metabolism
MH  - Male
MH  - Oleanolic Acid/administration & dosage
MH  - Olive Oil
MH  - Plant Oils/*administration & dosage
MH  - RNA, Messenger/genetics/metabolism
MH  - Triglycerides/*administration & dosage
MH  - Tumor Necrosis Factor-alpha/drug effects/metabolism
MH  - Young Adult
EDAT- 2011/06/18 06:00
MHDA- 2012/07/25 06:00
CRDT- 2011/06/18 06:00
PHST- 2011/01/24 00:00 [received]
PHST- 2011/06/01 00:00 [accepted]
PHST- 2011/06/18 06:00 [entrez]
PHST- 2011/06/18 06:00 [pubmed]
PHST- 2012/07/25 06:00 [medline]
AID - 10.1007/s00394-011-0215-2 [doi]
PST - ppublish
SO  - Eur J Nutr. 2012 Apr;51(3):301-9. doi: 10.1007/s00394-011-0215-2. Epub 2011 Jun 
      17.