PMID- 21682650
OWN - NLM
STAT- MEDLINE
DCOM- 20111209
LR  - 20151119
IS  - 1607-8888 (Electronic)
IS  - 1025-3890 (Linking)
VI  - 14
IP  - 5
DP  - 2011 Sep
TI  - Interleukin-18 expression in pig salivary glands and salivary content changes 
      during acute immobilization stress.
PG  - 549-56
LID - 10.3109/10253890.2011.565392 [doi]
AB  - Interleukin-18 (IL-18) has recently been considered a promising marker of stress 
      responses. In this study, to evaluate IL-18 as a noninvasive stress marker in 
      pigs, we investigated the expression of IL-18 in porcine salivary glands and its 
      presence in saliva, and its dynamics during acute immobilization stress in pigs. 
      IL-18 mRNA was detected robustly in the pig salivary glands by RT-PCR. 
      Immunohistochemical staining of IL-18 protein expression revealed that the 
      expression patterns differed among the three types of salivary glands (parotid, 
      submandibular, and sublingual gland). IL-18 was also detected in pig saliva by 
      ELISA, and a diurnal rhythm with a peak in the afternoon was observed. The IL-18 
      concentration in saliva was significantly increased during a 60-min acute 
      immobilization stress in thirteen 5-month-old pigs. These results are the first 
      evidence of a stress-related change of IL-18 in pig saliva. Salivary IL-18 may 
      thus become a useful noninvasive marker for the evaluation of acute stress in 
      pigs.
FAU - Muneta, Y
AU  - Muneta Y
AD  - Research Team for Advanced Biologicals, National Institute of Animal Health, 
      National Agriculture and Food Research Organization, 3-1-5, Kannondai, Tsukuba, 
      Ibaraki, Japan. ymuneta@affrc.go.jp
FAU - Minagawa, Y
AU  - Minagawa Y
FAU - Nakane, T
AU  - Nakane T
FAU - Shibahara, T
AU  - Shibahara T
FAU - Yoshikawa, T
AU  - Yoshikawa T
FAU - Omata, Y
AU  - Omata Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110619
PL  - England
TA  - Stress
JT  - Stress (Amsterdam, Netherlands)
JID - 9617529
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Circadian Rhythm
MH  - Female
MH  - Immobilization/psychology
MH  - Immunoglobulin A/metabolism
MH  - Immunohistochemistry
MH  - Interleukin-18/*biosynthesis/metabolism
MH  - Male
MH  - Saliva/*chemistry
MH  - Salivary Glands/*metabolism
MH  - Stress, Psychological/*physiopathology
MH  - Sus scrofa
EDAT- 2011/06/21 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/06/21 06:00
PHST- 2011/06/21 06:00 [entrez]
PHST- 2011/06/21 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - 10.3109/10253890.2011.565392 [doi]
PST - ppublish
SO  - Stress. 2011 Sep;14(5):549-56. doi: 10.3109/10253890.2011.565392. Epub 2011 Jun 
      19.