PMID- 21683678
OWN - NLM
STAT- MEDLINE
DCOM- 20111104
LR  - 20211020
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 416
IP  - 2
DP  - 2011 Sep 15
TI  - Chiral capillary electrophoresis-mass spectrometry of 3,4-dihydroxyphenylalanine: 
      evidence for its enantioselective metabolism in PC-12 nerve cells.
PG  - 191-5
LID - 10.1016/j.ab.2011.05.025 [doi]
AB  - A fully automated chiral capillary electrophoresis-tandem mass spectrometry 
      (CE-MS/MS) method was developed for enantiomeric quantification of 
      3,4-dihydroxyphenylalanine (DOPA) and its precursors, phenylalanine (Phe) and 
      tyrosine (Tyr). To avoid MS source contamination, a negatively charged chiral 
      selector, sulfated beta-cyclodextrin (sulfated beta-CD), that migrated away from the 
      detector was used in combination with the partial filling technique. The six 
      stereoisomers were simultaneously quantified in less than 12 min. Detection 
      limits were 0.48 and 0.51 muM for l- and d-DOPA enantiomers, respectively. Assay 
      reproducibility values (relative standard deviations [RSDs], n=6) were 4.43, 
      3.15, 4.91, 5.16, 3.96, and 3.25% for l- and d-DOPA, l- and d-Tyr, and l- and 
      d-Phe at 10 muM, respectively. Thanks to the high enantioseparation efficiency, 
      detection of trace d-DOPA in l-/d-DOPA mixtures could be achieved. The assay was 
      employed to study the metabolism of DOPA, a well-known therapeutic drug for 
      treating Parkinson's disease. It was found that l-DOPA was metabolized 
      effectively in PC-12 cells. Approximately 88% of l-DOPA disappeared after 
      incubation at a cell density of 2x10(6)cells/ml for 3 h. However, d-DOPA 
      coexisting with l-DOPA in the incubation solution remained intact. The 
      enantiospecific metabolism of DOPA in this neuronal model was demonstrated.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Yuan, Baiqing
AU  - Yuan B
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 
      39217, USA.
FAU - Wu, Hao
AU  - Wu H
FAU - Sanders, Talia
AU  - Sanders T
FAU - McCullum, Cassandra
AU  - McCullum C
FAU - Zheng, Yi
AU  - Zheng Y
FAU - Tchounwou, Paul B
AU  - Tchounwou PB
FAU - Liu, Yi-Ming
AU  - Liu YM
LA  - eng
GR  - G12 RR013459/RR/NCRR NIH HHS/United States
GR  - SC1 GM089557/GM/NIGMS NIH HHS/United States
GR  - SC1 GM089557-02/GM/NIGMS NIH HHS/United States
GR  - 2G12RR013459/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110526
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (beta-Cyclodextrins)
RN  - 42HK56048U (Tyrosine)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 63-84-3 (Dihydroxyphenylalanine)
RN  - JV039JZZ3A (betadex)
SB  - IM
MH  - Animals
MH  - Dihydroxyphenylalanine/*analysis/metabolism
MH  - Electrophoresis, Capillary/*methods
MH  - Mass Spectrometry/*methods
MH  - PC12 Cells
MH  - Phenylalanine/analysis
MH  - Rats
MH  - Stereoisomerism
MH  - Tyrosine/analysis
MH  - beta-Cyclodextrins/chemistry
PMC - PMC3138853
MID - NIHMS300319
EDAT- 2011/06/21 06:00
MHDA- 2011/11/05 06:00
PMCR- 2012/09/15
CRDT- 2011/06/21 06:00
PHST- 2011/03/18 00:00 [received]
PHST- 2011/05/11 00:00 [revised]
PHST- 2011/05/17 00:00 [accepted]
PHST- 2011/06/21 06:00 [entrez]
PHST- 2011/06/21 06:00 [pubmed]
PHST- 2011/11/05 06:00 [medline]
PHST- 2012/09/15 00:00 [pmc-release]
AID - S0003-2697(11)00333-2 [pii]
AID - 10.1016/j.ab.2011.05.025 [doi]
PST - ppublish
SO  - Anal Biochem. 2011 Sep 15;416(2):191-5. doi: 10.1016/j.ab.2011.05.025. Epub 2011 
      May 26.