PMID- 21683769
OWN - NLM
STAT- MEDLINE
DCOM- 20111214
LR  - 20131121
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 500
IP  - 1
DP  - 2011 Aug 1
TI  - Sexually dimorphic effects of estrogen on spines in cultures of accessory 
      olfactory bulb.
PG  - 77-81
LID - 10.1016/j.neulet.2011.06.005 [doi]
AB  - A sex difference has been reported in the responsiveness of the vomeronasal (VN) 
      system to pheromones. In the present study, to clarify a direct and acute 
      influence of 17beta-estradiol (E2) on the accessory olfactory bulb (AOB) neurons, we 
      investigated the effect of E2 on dendritic spines in cultured AOB cells derived 
      from male and female neonatal rats. After 17-18 days in vitro (DIV), cultured AOB 
      cells were transfected with GFP expression vectors. At 21-23 DIV, cells were 
      treated with E2, and time-lapse images of transfected AOB neurons identified as 
      granule cells were taken under a confocal laser scanning microscope for 3h. The 
      dendritic spine head area of granule cells was quantitatively evaluated, and 
      spine heads were classified into larger (>/= 1 mum(2)) and smaller (<1 mum(2)) ones 
      before E2-treatment (0 h). In cultured cells derived from both sexes, the larger 
      spines were not significantly changed at 1, 2 and 3 h after E2-treatment. In 
      contrast, E2-treatment significantly enlarged the head area of the smaller spines 
      of granule cells derived from the female, whereas E2 did not cause any 
      significant effects on those from the male. Our results provide evidence for the 
      sexually-dimorphic effect of E2 on spine development in AOB granule cells.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Wu, Yue
AU  - Wu Y
AD  - Department of Life Sciences, Toyo University, 1-1-1 Itakura, Oura, Gunma 
      374-0193, Japan.
FAU - Moriya-Ito, Keiko
AU  - Moriya-Ito K
FAU - Iwakura, Takashi
AU  - Iwakura T
FAU - Tsutiya, Atsuhiro
AU  - Tsutiya A
FAU - Ichikawa, Masumi
AU  - Ichikawa M
FAU - Ohtani-Kaneko, Ritsuko
AU  - Ohtani-Kaneko R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110613
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Estrogens)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Dendritic Spines/*drug effects
MH  - Estradiol/*pharmacology/physiology
MH  - Estrogens/*pharmacology/physiology
MH  - Female
MH  - Male
MH  - Neurons/*drug effects/ultrastructure
MH  - Olfactory Bulb/*ultrastructure
MH  - Rats
MH  - Rats, Wistar
MH  - *Sex Characteristics
EDAT- 2011/06/21 06:00
MHDA- 2011/12/15 06:00
CRDT- 2011/06/21 06:00
PHST- 2011/03/17 00:00 [received]
PHST- 2011/05/08 00:00 [revised]
PHST- 2011/06/03 00:00 [accepted]
PHST- 2011/06/21 06:00 [entrez]
PHST- 2011/06/21 06:00 [pubmed]
PHST- 2011/12/15 06:00 [medline]
AID - S0304-3940(11)00899-8 [pii]
AID - 10.1016/j.neulet.2011.06.005 [doi]
PST - ppublish
SO  - Neurosci Lett. 2011 Aug 1;500(1):77-81. doi: 10.1016/j.neulet.2011.06.005. Epub 
      2011 Jun 13.