PMID- 21685177
OWN - NLM
STAT- MEDLINE
DCOM- 20111205
LR  - 20211020
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 156
IP  - 4
DP  - 2011 Aug
TI  - Nuclear localization and interaction with COP1 are required for STO/BBX24 
      function during photomorphogenesis.
PG  - 1772-82
LID - 10.1104/pp.111.180208 [doi]
AB  - Arabidopsis (Arabidopsis thaliana) SALT TOLERANCE/B-BOX ZINC FINGER PROTEIN24 
      (STO/BBX24) is a negative regulator of the light signal transduction that 
      localizes to the nucleus of plant cells and interacts with CONSTITUTIVE 
      PHOTOMORPHOGENIC1 (COP1) in the yeast (Saccharomyces cerevisiae) two-hybrid 
      system. The protein contains two B-box zinc-finger motives at the N terminus and 
      a conserved motif at the C-terminal part required for the interaction with COP1. 
      BBX24 accumulates during deetiolation of young seedlings in the first hours of 
      exposure to light. However, this accumulation is transient and decreases after 
      prolonged light irradiation. Here, we identified the amino acidic residues 
      necessary for the nuclear import of the protein. In addition, we created mutated 
      forms of the protein, and analyzed them by overexpression in the bbx24-1 mutant 
      background. Our results indicate that the degradation of BBX24 occurs, or at 
      least is initiated in the nucleus, and this nuclear localization is a 
      prerequisite to fulfill its function in light signaling. Moreover, mutations in 
      the region responsible for the interaction with COP1 revealed that a physical 
      interaction of the proteins is also required for degradation of BBX24 in the 
      light and for normal photomorphogenesis.
FAU - Yan, Huili
AU  - Yan H
AD  - Faculty of Biology, Cell Biology, University of Freiburg, D-79104 Freiburg, 
      Germany.
FAU - Marquardt, Katrin
AU  - Marquardt K
FAU - Indorf, Martin
AU  - Indorf M
FAU - Jutt, Dominic
AU  - Jutt D
FAU - Kircher, Stefan
AU  - Kircher S
FAU - Neuhaus, Gunther
AU  - Neuhaus G
FAU - Rodriguez-Franco, Marta
AU  - Rodriguez-Franco M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110617
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (BBX24 protein, Arabidopsis)
RN  - 0 (Mutant Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Repressor Proteins)
RN  - 0 (STO protein, Arabidopsis)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/genetics/*growth & development/*radiation effects
MH  - Arabidopsis Proteins/*metabolism
MH  - Cell Nucleus/*metabolism/radiation effects
MH  - Green Fluorescent Proteins/metabolism
MH  - *Light
MH  - Morphogenesis/*radiation effects
MH  - Mutant Proteins/metabolism
MH  - Nuclear Localization Signals/metabolism
MH  - Plants, Genetically Modified
MH  - Protein Binding/radiation effects
MH  - Protein Transport/radiation effects
MH  - Proteolysis/radiation effects
MH  - Repressor Proteins/*metabolism
MH  - Sequence Deletion
MH  - Subcellular Fractions/metabolism
MH  - Ubiquitin-Protein Ligases/*metabolism
PMC - PMC3149933
EDAT- 2011/06/21 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/08/01
CRDT- 2011/06/21 06:00
PHST- 2011/06/21 06:00 [entrez]
PHST- 2011/06/21 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - pp.111.180208 [pii]
AID - 180208 [pii]
AID - 10.1104/pp.111.180208 [doi]
PST - ppublish
SO  - Plant Physiol. 2011 Aug;156(4):1772-82. doi: 10.1104/pp.111.180208. Epub 2011 Jun 
      17.