PMID- 21685826
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20110727
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 92
IP  - 3
DP  - 2011 Aug 15
TI  - Epstein-barr virus gene expression, human leukocyte antigen alleles and chronic 
      high viral loads in pediatric renal transplant patients.
PG  - 328-33
LID - 10.1097/TP.0b013e3182247bf2 [doi]
AB  - BACKGROUND: Studies have identified solid organ transplant recipients who remain 
      asymptomatic despite maintaining chronic high Epstein-Barr virus (EBV) viral 
      loads. We examined clinical manifestations, EBV gene expression, human leukocyte 
      antigen (HLA) alleles, and specific T-cell responses to EBV infection in 
      pediatric renal transplant patients. METHODS: Seventeen pediatric renal 
      transplant patients were categorized according to EBV viral load into those with 
      chronic high viral loads (CHL) and recipients who resolve EBV infection (REI). 
      EBV gene expression was analyzed using real-time PCR assays and EBV-specific T 
      cells were analyzed by flow cytometry. RESULTS: EBV gene, EBV-encoded small RNA 
      1, was expressed at significantly higher levels in CHL compared with EBV 
      seropositive controls (P=0.005) and raised compared with REI. BamHI A right-ward 
      transcripts were also expressed at higher levels in CHL patients (P=0.03) than in 
      REI. Expression of latent genes, EBNA1, LMP1, LMP2, and lytic gene BZLF1 were 
      restricted to the CHL group with viral gene expression varying over time. 
      HLA-A*02 allele expression was predominant in CHL patients (80%) and GLC 
      lytic-specific cytotoxic T-lymphocytes were absent. In contrast, HLA-B*08 allele 
      expression was prevalent in REI patients (71%) and RAK lytic cytotoxic 
      T-lymphocytes were detected in all patients. CONCLUSION: EBV gene expression in 
      CHL carriers differs from those that resolve infection and should be interpreted 
      alongside HLA polymorphisms.
FAU - Moran, Julie
AU  - Moran J
AD  - Centre for Research in Infectious Diseases, School of Medicine and Medical 
      Science, University College Dublin, Belfield, Dublin, Ireland.
FAU - Carr, Michael
AU  - Carr M
FAU - Waters, Allison
AU  - Waters A
FAU - Boyle, Sheila
AU  - Boyle S
FAU - Riordan, Michael
AU  - Riordan M
FAU - Connell, Jeff
AU  - Connell J
FAU - Awan, Atif
AU  - Awan A
FAU - Hall, William
AU  - Hall W
FAU - Hassan, Jaythoon
AU  - Hassan J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (HLA Antigens)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adolescent
MH  - CD8-Positive T-Lymphocytes/immunology/virology
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Epstein-Barr Virus Infections/*genetics/*immunology
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Viral
MH  - HLA Antigens/*genetics/immunology
MH  - Herpesvirus 4, Human/*genetics
MH  - Humans
MH  - Infant
MH  - *Kidney Transplantation
MH  - Male
MH  - Postoperative Complications/genetics/immunology/virology
MH  - RNA, Viral/genetics
MH  - Recurrence
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Viral Load/*genetics/immunology
EDAT- 2011/06/21 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/06/21 06:00
PHST- 2011/06/21 06:00 [entrez]
PHST- 2011/06/21 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 10.1097/TP.0b013e3182247bf2 [doi]
PST - ppublish
SO  - Transplantation. 2011 Aug 15;92(3):328-33. doi: 10.1097/TP.0b013e3182247bf2.