PMID- 2168860
OWN - NLM
STAT- MEDLINE
DCOM- 19901018
LR  - 20191029
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 180
IP  - 4-5
DP  - 1990 Jun
TI  - Modulation of Forssman glycosphingolipid expression by murine macrophages: 
      coinduction with class II MHC antigen by the lymphokines IL4 and IL6.
PG  - 405-18
AB  - In contrast to murine spleen M phi, resident peritoneal M phi from health mice 
      express very little Forssman glycolipid antigen (Fo). The following experiments 
      suggest that Fo expression by peritoneal M phi may be associated with 
      inflammation. Balb/c and CBA/J mice were given inflammatory stimuli by i.p. 
      injection of live BCG, thioglycollate (TG), Corynebacterium parvum (CP), proteose 
      peptone (PP), or LPS. Control animals received pyrogen-free saline. Expression of 
      Fo and Ia antigen by peritoneal M phi was determined by immunofluorescence after 
      4 d. Application of TG or CP led to an up to 30-fold increase in Fo+, Ia+ double 
      positive M phi over that in control animals. LPS caused mainly an increase in the 
      percentage of double-positive M phi, whereas no effects were seen in BCG or PP 
      treated animals. To clarify the possible involvement of cytokines in this process 
      and to identify these, the effects of LPS and various cytokines on in vitro 
      induction of Fo and Ia expression were studied in further experiments. LPS, IL6, 
      and IL4 caused induction of up to 15% Fo+ and Ia+ M phi after a 4 d culture 
      period. M phi colony stimulating factor (M-CSF) from lung-conditioned medium was 
      also moderately active. IL1, TNF, and IL2 had no influence, whereas IFN-gamma 
      only induced Ia. For a successful in vitro induction of Fo and Ia, a prior 
      priming of the mice with PP appeared mandatory. This suggests that only M phi of 
      a certain developmental stage can acquire Fo under the influence of the 
      appropriate cytokines. The data may provide the first evidence for 
      cytokine-mediated modulation of a glycolipid antigen of known chemical structure.
FAU - von Kleist, R
AU  - von Kleist R
AD  - Immunobiology Research Group, Gesellschaft fur Biotechnologische Forschung, 
      Braunschweig, FRG.
FAU - Schmitt, E
AU  - Schmitt E
FAU - Westermann, J
AU  - Westermann J
FAU - Muhlradt, P F
AU  - Muhlradt PF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Antigens, Heterophile)
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Globosides)
RN  - 0 (Glycosphingolipids)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 207137-56-2 (Interleukin-4)
RN  - 60267-39-2 (Forssman glycolipid)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 9013-60-9 (Forssman Antigen)
SB  - IM
MH  - Animals
MH  - Antigens, Heterophile/*biosynthesis
MH  - Colony-Stimulating Factors/physiology
MH  - Female
MH  - Forssman Antigen/*biosynthesis
MH  - Globosides/*biosynthesis
MH  - Glycosphingolipids/*biosynthesis
MH  - Histocompatibility Antigens Class II/*biosynthesis
MH  - Interleukin-4/*physiology
MH  - Interleukin-6/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Colony-Stimulating Factor
MH  - Macrophages/drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Peritoneal Cavity/cytology
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
AID - S0171-2985(11)80302-5 [pii]
AID - 10.1016/s0171-2985(11)80302-5 [doi]
PST - ppublish
SO  - Immunobiology. 1990 Jun;180(4-5):405-18. doi: 10.1016/s0171-2985(11)80302-5.