PMID- 21691724
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20240426
IS  - 1432-0851 (Electronic)
IS  - 0340-7004 (Print)
IS  - 0340-7004 (Linking)
VI  - 60
IP  - 11
DP  - 2011 Nov
TI  - Poly(I:C)-induced tumour cell death leads to DC maturation and Th1 activation.
PG  - 1609-24
LID - 10.1007/s00262-011-1058-7 [doi]
AB  - Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class 
      I molecules derived from apoptotic tumour cells for direct recognition by 
      cytotoxic T cells. This function has lead to DCs being used in vaccine 
      strategies. In this study, we investigate the effect of inducing apoptosis in 
      tumour cell lines using IFN-gamma and poly(I:C), the subsequent maturation of the 
      endocytosing DC and its ability to direct the resulting T cell response. We show 
      that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation 
      and activation with a Th1 cell polarising capacity. In contrast, these effects 
      are not seen by DCs loaded with gamma-irradiated apoptotic tumour cells. We propose 
      that the manner in which tumour cells are induced to die can have a profound 
      effect on the endocytosing DC and the resulting T cell response.
FAU - Kovalcsik, Edit
AU  - Kovalcsik E
AD  - Systems Immunology Group, Cellular and Molecular Medicine, St George's University 
      of London, UK.
FAU - Lowe, Katie
AU  - Lowe K
FAU - Fischer, Mike
AU  - Fischer M
FAU - Dalgleish, Angus
AU  - Dalgleish A
FAU - Bodman-Smith, Mark D
AU  - Bodman-Smith MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110621
PL  - Germany
TA  - Cancer Immunol Immunother
JT  - Cancer immunology, immunotherapy : CII
JID - 8605732
RN  - 0 (Cytokines)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Apoptosis/immunology
MH  - Cell Death/immunology
MH  - Cell Line, Tumor
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology/pathology
MH  - Humans
MH  - Major Histocompatibility Complex/immunology
MH  - Melanoma/*immunology/pathology
MH  - Poly I-C/immunology/*pharmacology
MH  - T-Lymphocytes/*immunology/metabolism
MH  - Th1 Cells/*immunology
PMC - PMC11028976
EDAT- 2011/06/22 06:00
MHDA- 2012/02/14 06:00
PMCR- 2011/06/21
CRDT- 2011/06/22 06:00
PHST- 2010/11/12 00:00 [received]
PHST- 2011/05/30 00:00 [accepted]
PHST- 2011/06/22 06:00 [entrez]
PHST- 2011/06/22 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
PHST- 2011/06/21 00:00 [pmc-release]
AID - 1058 [pii]
AID - 10.1007/s00262-011-1058-7 [doi]
PST - ppublish
SO  - Cancer Immunol Immunother. 2011 Nov;60(11):1609-24. doi: 
      10.1007/s00262-011-1058-7. Epub 2011 Jun 21.