PMID- 21692167
OWN - NLM
STAT- MEDLINE
DCOM- 20120801
LR  - 20131121
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 27
IP  - 1
DP  - 2012 Jan-Feb
TI  - A terbium-sensitized spectrofluorimetric method for determination of 
      catecholamines in a serum sample with micelle medium.
PG  - 84-90
LID - 10.1002/bio.1332 [doi]
AB  - A terbium-sensitized spectrofluorimetric method has been developed for 
      determination of catecholamines such as norepinephrine (NE), epinephrine (EP) and 
      dopamine (DA), using sodium dodecyl benzene sulphonate (SDBS). Fluorescence 
      sensitization of terbium ions (Tb(3+) ) by complexation with catecholamines in 
      the presence of SDBS was observed. The fluorescence intensities of the Tb(3+) 
      -catecholamine complexes were highly enhanced by introducing SDBS with an 
      emission maximum at 545 nm after excitation at 290 nm. The conditions for the 
      complex formation of Tb(3+) -catecholamine were investigated systematically and 
      optimized to determine catecholamines in a serum sample. Under the optimum 
      conditions, the fluorescence intensities of the Tb(3+) -catecholamine complexes 
      were increased linearly with the concentration of NE, EP and DA over the ranges 
      2.5 x 10(-10) -1.0 x 10(-8) , 2.5 x 10(-10) -1.0 x 10(-8) and 2.5 x 10(-9) 
      -1.0 x 10(-7)  g/mL with correlation coefficients of 0.999, 0.999 and 0.9996, 
      respectively. The limits of detection (3delta) of NE, EP and DA were found to be 
      4.6 x 10(-11) , 7.8 x 10(-11) and 8.38 x 10(-10)  g/mL, respectively. Precision 
      of the method was tested at the concentration level of 1.2 x 10(-7)  g/mL for 
      five replicate measurements of NE, EP and DA, giving relative standard deviations 
      (RSDs) of 1.41%, 1.23% and 1.89%, respectively. The interaction mechanism of the 
      Tb(3+) -catecholamine complexes system was investigated and presented with 
      ultraviolet absorption spectra. The proposed method has been applied for the 
      quantitative determination of NE, EP and DA in a spiked serum sample and a 
      pharmaceutical preparation sample.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Kamruzzaman, Mohammad
AU  - Kamruzzaman M
AD  - Department of Chemistry, Kyungpook National University, Daegu, South Korea.
FAU - Alam, Al-Mahmnur
AU  - Alam AM
FAU - Lee, Sang Hak
AU  - Lee SH
FAU - Kim, Young Ho
AU  - Kim YH
FAU - Kim, Sung Hong
AU  - Kim SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110621
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Catecholamines)
RN  - 0 (Micelles)
RN  - 06SSF7P179 (Terbium)
SB  - IM
MH  - Catecholamines/analysis/*blood
MH  - Limit of Detection
MH  - Micelles
MH  - Reproducibility of Results
MH  - Spectrometry, Fluorescence/*methods/standards
MH  - *Terbium
EDAT- 2011/06/22 06:00
MHDA- 2012/08/02 06:00
CRDT- 2011/06/22 06:00
PHST- 2011/01/05 00:00 [received]
PHST- 2011/04/11 00:00 [revised]
PHST- 2011/05/18 00:00 [accepted]
PHST- 2011/06/22 06:00 [entrez]
PHST- 2011/06/22 06:00 [pubmed]
PHST- 2012/08/02 06:00 [medline]
AID - 10.1002/bio.1332 [doi]
PST - ppublish
SO  - Luminescence. 2012 Jan-Feb;27(1):84-90. doi: 10.1002/bio.1332. Epub 2011 Jun 21.