PMID- 2169269
OWN - NLM
STAT- MEDLINE
DCOM- 19901019
LR  - 20190820
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 5
IP  - 3
DP  - 1990 Sep
TI  - Brain-derived neurotrophic factor increases survival and differentiated functions 
      of rat septal cholinergic neurons in culture.
PG  - 297-306
AB  - Brain-derived neurotrophic factor (BDNF) was found to promote the survival of E17 
      rat embryo septal cholinergic neurons in culture, as assessed by a histochemical 
      stain for acetylcholinesterase (AChE). A 2.4-fold increase in neuronal survival 
      was achieved with 10 ng/ml BDNF. After initial deprivation of growth factor for 7 
      days, BDNF failed to bring about this increase, strongly suggesting that BDNF 
      promotes cell survival and not just induction of AChE. BDNF was also found to 
      increase the levels of cholinergic enzymes; choline acetyltransferase (ChAT) and 
      AChE activities were increased by approximately 2-fold in the presence of 50 
      ng/ml BDNF. BDNF produced a 3-fold increase in the number of cells bearing the 
      NGF receptor, as detected by the monoclonal antibody IgG-192. Although NGF had no 
      additive effect with BDNF in terms of neuronal survival, suggesting that both act 
      on a similar neuronal population, the combination of both produced an additive 
      response, approximately a 6-fold increase, in ChAT activity.
FAU - Alderson, R F
AU  - Alderson RF
AD  - Regeneron Pharmaceuticals Inc., Tarrytown, New York 10591.
FAU - Alterman, A L
AU  - Alterman AL
FAU - Barde, Y A
AU  - Barde YA
FAU - Lindsay, R M
AU  - Lindsay RM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Nerve Growth Factors/metabolism/pharmacology
MH  - Nerve Tissue Proteins/metabolism/*pharmacology
MH  - Neurons/enzymology/metabolism/*physiology
MH  - Parasympathetic Nervous System/cytology/*physiology
MH  - Rats
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, Nerve Growth Factor
MH  - Septum Pellucidum/cytology/*physiology
MH  - Time Factors
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
AID - 0896-6273(90)90166-D [pii]
AID - 10.1016/0896-6273(90)90166-d [doi]
PST - ppublish
SO  - Neuron. 1990 Sep;5(3):297-306. doi: 10.1016/0896-6273(90)90166-d.