PMID- 2169499
OWN - NLM
STAT- MEDLINE
DCOM- 19901019
LR  - 20190510
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 162
IP  - 4
DP  - 1990 Oct
TI  - Variations in the replication and antigenic properties of human herpesvirus 6 
      strains.
PG  - 852-7
AB  - The Z29 and U1102 strains of human herpesvirus 6 (HHV-6) were compared for their 
      ability to replicate in fresh peripheral blood lymphocytes (PBL) and in 
      continuous T cell lines. The replication of both strains in PBL was enhanced by 
      mitogenic activation of cell growth. U1102 replicated in the continuous T cell 
      lines, J JHAN and HSB-2, whereas no Z29 replication was observed in these cell 
      lines as judged by infectious virus yields, the presence of viral antigens, and 
      viral DNA replication. The two strains were compared with respect to their 
      ability to react in immunofluorescence assays with monoclonal antibodies (MAbs) 
      prepared against the GS strain of HHV-6. These MAbs are directed against six 
      different polypeptides including three glycoproteins. All MAbs reacted with cells 
      infected with the U1102 strain. The Z29-infected cells reacted with four MAbs but 
      failed to react with MAbs specific for an 82- to 105-kDa major surface 
      glycoprotein and with one MAb reactive with a nonglycosylated 180-kDa protein. 
      Taken together, the two strains of HHV-6 exhibit variations with regard to their 
      growth and antigenic properties.
FAU - Wyatt, L S
AU  - Wyatt LS
AD  - Laboratory of Viral Diseases, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Rockville, Maryland 20852.
FAU - Balachandran, N
AU  - Balachandran N
FAU - Frenkel, N
AU  - Frenkel N
LA  - eng
GR  - AI-24224/AI/NIAID NIH HHS/United States
GR  - RR-05373-28/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antigenic Variation
MH  - Antigens, Viral/analysis/*immunology
MH  - Blotting, Southern
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytopathogenic Effect, Viral
MH  - DNA Replication
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 6, Human/genetics/immunology/*physiology
MH  - Humans
MH  - Lymphocytes/*microbiology
MH  - T-Lymphocytes/*microbiology
MH  - Virus Replication
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.1093/infdis/162.4.852 [doi]
PST - ppublish
SO  - J Infect Dis. 1990 Oct;162(4):852-7. doi: 10.1093/infdis/162.4.852.