PMID- 21695528
OWN - NLM
STAT- MEDLINE
DCOM- 20111121
LR  - 20211020
IS  - 1432-203X (Electronic)
IS  - 0721-7714 (Linking)
VI  - 30
IP  - 9
DP  - 2011 Sep
TI  - Fluorescence in situ hybridization on plant extended chromatin DNA fibers for 
      single-copy and repetitive DNA sequences.
PG  - 1779-86
LID - 10.1007/s00299-011-1086-y [doi]
AB  - The compactness of plant chromosomes and the structure of the plant cell wall and 
      cytoplasm provide a great obstacle to fluorescence in situ hybridization (FISH) 
      for single-copy or low-copy DNA sequences. Consequently, many new methods for 
      improving spatial resolution via chromosomal stretching have been employed to 
      overcome this technical challenge. In this article, a technique for extracting 
      cell-wall free nuclei at mitotic interphase, then using these nuclei to prepare 
      extended DNA fibers (EDFs) by the method of a receding interface, whereby 
      slide-mounted chromatin produces EDFs in concert with gravity-assisted buffer 
      flow, was adopted as a result of the low frequency of EDF damage produced by this 
      procedure. To examine the quality of these EDFs, we used single-copy gene 
      encoding S-locus receptor kinase and multi-copy 5S rDNA (ribosomal DNA) as 
      probes. The resulting EDFs proved suitable for high-resolution FISH mapping for 
      repetitive DNA sequences, and the localization of a single-copy locus.
FAU - Yang, Kun
AU  - Yang K
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
FAU - Zhang, Hecui
AU  - Zhang H
FAU - Converse, Richard
AU  - Converse R
FAU - Wang, Yong
AU  - Wang Y
FAU - Rong, Xiaoying
AU  - Rong X
FAU - Wu, Zhigang
AU  - Wu Z
FAU - Luo, Bing
AU  - Luo B
FAU - Xue, Liyan
AU  - Xue L
FAU - Jian, Li
AU  - Jian L
FAU - Zhu, Liquan
AU  - Zhu L
FAU - Wang, Xiaojia
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110622
PL  - Germany
TA  - Plant Cell Rep
JT  - Plant cell reports
JID - 9880970
RN  - 0 (Chromatin)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (S-receptor kinase)
SB  - IM
MH  - Brassica/genetics
MH  - Cell Nucleus/genetics
MH  - Cell Wall/metabolism
MH  - Chromatin/metabolism
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Plant/genetics
MH  - DNA Probes/genetics/metabolism
MH  - DNA, Plant/*genetics
MH  - Flowers/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Interphase
MH  - Metaphase
MH  - Pachytene Stage
MH  - Plant Proteins/genetics
MH  - Plant Roots/genetics
MH  - Protein Kinases/genetics
MH  - RNA, Ribosomal, 5S/genetics/metabolism
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2011/06/23 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/06/23 06:00
PHST- 2011/02/23 00:00 [received]
PHST- 2011/05/06 00:00 [accepted]
PHST- 2011/05/05 00:00 [revised]
PHST- 2011/06/23 06:00 [entrez]
PHST- 2011/06/23 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1007/s00299-011-1086-y [doi]
PST - ppublish
SO  - Plant Cell Rep. 2011 Sep;30(9):1779-86. doi: 10.1007/s00299-011-1086-y. Epub 2011 
      Jun 22.