PMID- 21696466
OWN - NLM
STAT- MEDLINE
DCOM- 20111202
LR  - 20240109
IS  - 1365-2958 (Electronic)
IS  - 0950-382X (Linking)
VI  - 81
IP  - 4
DP  - 2011 Aug
TI  - Simultaneous silencing of endo-beta-1,4 xylanase genes reveals their roles in the 
      virulence of Magnaporthe oryzae.
PG  - 1008-19
LID - 10.1111/j.1365-2958.2011.07746.x [doi]
AB  - Due to functional redundancy, it is often difficult to genetically analyse the 
      biological function of fungal cell wall-degrading enzymes that belong to a gene 
      family. To overcome this difficulty, we used RNAi to knock-down (KD) multiple 
      xylanase genes to elucidate their roles in the pathogenicity of the blast fungus, 
      Magnaporthe oryzae. To obtain the maximum average efficiency of gene silencing 
      for the xylanase genes, we used the 'building blocks method', in which a 40 bp 
      sequence was chosen from an endoxylanase gene, and 10 such sequences from 10 
      endoxylanases were combined to make an artificial RNAi trigger by synthetic DNA. 
      Quantitative RT-PCR analysis revealed that the transcript levels of all the 
      expressed xylanase genes were significantly reduced in KD mutants with the 
      artificial RNAi trigger. Even though the KD mutants did not completely lose their 
      pathogenicity to host plants, the number of lesions, rate of penetration and 
      extent of infected cells were all reduced in KD mutant-infected leaves. The 
      degree of pathogenicity reduction was associated with the silencing levels of 
      xylanase mRNA and enzymatic activity in the KD mutants. Cytological analysis 
      indicated that xylanases play significant roles in both vertical penetration and 
      horizontal expansion of M. oryzae in infected plants.
CI  - (c) 2011 Blackwell Publishing Ltd.
FAU - Nguyen, Quoc Bao
AU  - Nguyen QB
AD  - Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe 
      University, 1-1 Rokkodai-cho, Nada 657-8501 Kobe, Japan.
FAU - Itoh, Kenji
AU  - Itoh K
FAU - Van Vu, Ba
AU  - Van Vu B
FAU - Tosa, Yukio
AU  - Tosa Y
FAU - Nakayashiki, Hitoshi
AU  - Nakayashiki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110711
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
RN  - 0 (Virulence Factors)
RN  - EC 3.2.1.8 (Endo-1,4-beta Xylanases)
SB  - IM
MH  - Endo-1,4-beta Xylanases/genetics/*metabolism
MH  - Gene Knockdown Techniques
MH  - Gene Silencing
MH  - Magnaporthe/*enzymology/*pathogenicity
MH  - Plant Diseases/*microbiology
MH  - Virulence
MH  - Virulence Factors/genetics/*metabolism
EDAT- 2011/06/24 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/06/24 06:00
PHST- 2011/06/24 06:00 [entrez]
PHST- 2011/06/24 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1111/j.1365-2958.2011.07746.x [doi]
PST - ppublish
SO  - Mol Microbiol. 2011 Aug;81(4):1008-19. doi: 10.1111/j.1365-2958.2011.07746.x. 
      Epub 2011 Jul 11.