PMID- 21696775
OWN - NLM
STAT- MEDLINE
DCOM- 20120713
LR  - 20131121
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 175
IP  - 2
DP  - 2012 Jun 15
TI  - Cyclosporin-A does not prevent cold ischemia/reperfusion injury of rat livers.
PG  - 333-42
LID - 10.1016/j.jss.2011.04.018 [doi]
AB  - Cyclosporin-A (CsA) has been reported to protect livers from warm 
      ischemia/reperfusion (I/R) injury. To study if CsA has also a protective effect 
      on cold I/R injury, two models were used: the isolated perfused rat liver (IPRL) 
      and the orthotopic rat liver transplantation (ORLT). (1) IPRL: Livers were 
      preserved for 24 h (5 degrees C) in University of Wisconsin (UW) solution alone (group 
      1), with CsA (400 nM) dissolved in dimethylsulfoxide (50 muM) (group 2), and with 
      dimethylsulfoxide (DMSO) alone (group 3). Livers were reperfused for 60 min 
      (37 degrees C) (n = 8/group). Cell necrosis was evaluated by trypan blue uptake and 
      apoptosis by laddering and terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) assay, and by caspase-3 activation. Marked and similar 
      sinusoidal endothelial cell necrosis was found in the three groups while 
      apoptosis was found similarly deceased in groups 2 and 3 compared with group 1. 
      (2) ORLT: Donors received either CsA (5 mg/kg) or corn oil 24 h before 
      transplantation. Recipients were sacrificed after 240 min; cell necrosis and 
      apoptosis were then evaluated. No difference was found between treated and 
      control groups. The current data strongly suggest that CsA has no protective 
      effect on hepatic cold I/R injury. Hepatocyte apoptosis can be reduced by 
      antioxidants, as occurred with DMSO, but introduction of CsA does not provide 
      additional protective effect.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Tarrab, Esther
AU  - Tarrab E
AD  - Centre de Recherche, Centre Hospitalier, Universite de Montreal, Hopital Saint 
      Luc, Montreal, Quebec, Canada.
FAU - Huet, Pierre-Michel
AU  - Huet PM
FAU - Brault, Antoine
AU  - Brault A
FAU - Rocheleau, Bernard
AU  - Rocheleau B
FAU - Laurens, Marina
AU  - Laurens M
FAU - Crenesse, Dominique
AU  - Crenesse D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110505
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Insulin)
RN  - 0 (Organ Preservation Solutions)
RN  - 0 (University of Wisconsin-lactobionate solution)
RN  - 63CZ7GJN5I (Allopurinol)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - EC 3.4.22.- (Caspase 3)
RN  - GAN16C9B8O (Glutathione)
RN  - K72T3FS567 (Adenosine)
RN  - N5O3QU595M (Raffinose)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
CIN - J Surg Res. 2012 Jul;176(1):e1-3. PMID: 22575360
MH  - Adenosine/pharmacology
MH  - Allopurinol/pharmacology
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caspase 3/metabolism
MH  - *Cold Ischemia
MH  - Cyclosporine/pharmacology/*therapeutic use
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Endothelium/drug effects/pathology
MH  - Glutathione/pharmacology
MH  - Hepatocytes/drug effects/pathology
MH  - Insulin/pharmacology
MH  - Liver/*blood supply/enzymology/*pathology
MH  - Liver Transplantation/pathology
MH  - Male
MH  - Models, Animal
MH  - Organ Preservation Solutions/pharmacology
MH  - Raffinose/pharmacology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Regional Blood Flow/physiology
MH  - Reperfusion Injury/enzymology/*pathology/*prevention & control
EDAT- 2011/06/24 06:00
MHDA- 2012/07/14 06:00
CRDT- 2011/06/24 06:00
PHST- 2011/01/18 00:00 [received]
PHST- 2011/03/28 00:00 [revised]
PHST- 2011/04/07 00:00 [accepted]
PHST- 2011/06/24 06:00 [entrez]
PHST- 2011/06/24 06:00 [pubmed]
PHST- 2012/07/14 06:00 [medline]
AID - S0022-4804(11)00369-6 [pii]
AID - 10.1016/j.jss.2011.04.018 [doi]
PST - ppublish
SO  - J Surg Res. 2012 Jun 15;175(2):333-42. doi: 10.1016/j.jss.2011.04.018. Epub 2011 
      May 5.