PMID- 21700269
OWN - NLM
STAT- MEDLINE
DCOM- 20120228
LR  - 20161125
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 56
IP  - 11
DP  - 2011 Nov
TI  - Effect of PGE(2) induced by compressive and tensile stresses on cementoblast 
      differentiation in vitro.
PG  - 1238-46
LID - 10.1016/j.archoralbio.2011.05.007 [doi]
AB  - OBJECTIVE: The aim of the study was to clarify the mechanisms underlying 
      orthodontically induced root resorption by characterizing the role of PGE(2) 
      induced by compressive stress (CS) and tensile stress (TS) on cementoblast 
      metabolism in vitro. DESIGN: Mouse cementoblast cell line OCCM-30 was 
      continuously stimulated with 0.2 KPa CS or 5.0 KPa TS. COX-2 mRNA expression and 
      PGE(2) production were thus quantified. In addition, cells were treated with 
      COX-2 inhibitor and the role of PGE(2) induced by CS or TS on the expression of 
      genes related to cementoblast differentiation was examined. PGE(2) receptors mRNA 
      expression induced by CS or TS was also evaluated. Moreover, cells were treated 
      with exogenous PGE(2) and the role of PGE(2) concentration on matrix 
      mineralization was verified. RESULTS: CS and TS enhanced COX-2 mRNA expression 
      and PGE(2) production. PGE(2) synthesis, however, was markedly induced by CS. 
      Gene expression of bone morphogenetic protein 2 (BMP-2), osteocalcin (OCN) and 
      receptor activator for nuclear factor kappaB ligand (RANKL) was enhanced by CS on 
      an endogenous PGE(2)-mediated manner. Osteoprotegerin (OPG) expression was not 
      affected by CS. Meanwhile, TS up-regulated the expression of BMP-2 and alkaline 
      phosphatase (ALP) on an endogenous PGE(2)-mediated manner. TS down-regulated 
      RANKL mRNA expression, whilst OPG expression was not affected. Moreover, EP4 mRNA 
      expression was considerably enhanced by TS. Regarding PGE(2) concentration, only 
      cells treated with low concentration presented anabolic response. CONCLUSIONS: 
      Gene expression was differentially regulated according to the type of mechanical 
      stimulation applied to cementoblasts. In addition, it is shown that PGE(2) plays 
      an important role on mediating cementoblast mechanosensitivity.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Rego, Emanuel Braga
AU  - Rego EB
AD  - Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima 
      University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, 
      Hiroshima 734-8553, Japan. emanuelbraga@hotmail.com
FAU - Inubushi, Toshihiro
AU  - Inubushi T
FAU - Kawazoe, Aki
AU  - Kawazoe A
FAU - Miyauchi, Mutsumi
AU  - Miyauchi M
FAU - Tanaka, Eiji
AU  - Tanaka E
FAU - Takata, Takashi
AU  - Takata T
FAU - Tanne, Kazuo
AU  - Tanne K
LA  - eng
PT  - Journal Article
DEP - 20110622
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 2/biosynthesis
MH  - Cell Differentiation/genetics
MH  - Cell Line, Transformed
MH  - Cementogenesis/*genetics
MH  - Cyclooxygenase 2/biosynthesis
MH  - Dental Cementum/*cytology/*metabolism/physiology
MH  - *Dental Stress Analysis
MH  - Dinoprostone/biosynthesis/*physiology
MH  - Gene Expression Regulation
MH  - *Mechanotransduction, Cellular
MH  - Mice
MH  - Mice, Transgenic
MH  - Osteocalcin/biosynthesis
MH  - Physical Stimulation
MH  - RANK Ligand/biosynthesis
MH  - Receptors, G-Protein-Coupled/biosynthesis/physiology
MH  - Root Resorption/etiology
MH  - Stress, Mechanical
MH  - Tooth Movement Techniques/adverse effects
EDAT- 2011/06/28 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/06/25 06:00
PHST- 2011/01/14 00:00 [received]
PHST- 2011/05/17 00:00 [revised]
PHST- 2011/05/17 00:00 [accepted]
PHST- 2011/06/25 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - S0003-9969(11)00161-0 [pii]
AID - 10.1016/j.archoralbio.2011.05.007 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2011 Nov;56(11):1238-46. doi: 10.1016/j.archoralbio.2011.05.007. 
      Epub 2011 Jun 22.