PMID- 21700889
OWN - NLM
STAT- MEDLINE
DCOM- 20120111
LR  - 20130926
IS  - 1522-1601 (Electronic)
IS  - 0161-7567 (Linking)
VI  - 111
IP  - 3
DP  - 2011 Sep
TI  - Bronchoconstriction in nonhuman primates: a species comparison.
PG  - 791-8
LID - 10.1152/japplphysiol.00162.2011 [doi]
AB  - Bronchoconstriction is a characteristic symptom of various chronic obstructive 
      respiratory diseases such as chronic obstructive pulmonary disease and asthma. 
      Precision-cut lung slices (PCLS) are a suitable ex vivo model to study 
      physiological mechanisms of bronchoconstriction in different species. In the 
      present study, we established an ex vivo model of bronchoconstriction in nonhuman 
      primates (NHPs). PCLS prepared from common marmosets, cynomolgus macaques, rhesus 
      macaques, and anubis baboons were stimulated with increasing concentrations of 
      representative bronchoconstrictors: methacholine, histamine, serotonin, 
      leukotriene D(4) (LTD(4)), U46619, and endothelin-1. Alterations in the airway 
      caliber were measured and compared with previously published data from rodents, 
      guinea pigs, and humans. Methacholine induced maximal airway constriction, 
      varying between 74 and 88% in all NHP species, whereas serotonin was ineffective. 
      Histamine induced maximal bronchoconstriction of 77 to 90% in rhesus macaques, 
      cynomolgus macaques, and baboons and a lesser constriction of 53% in marmosets. 
      LTD(4) was ineffective in marmosets and rhesus macaques but induced a maximum 
      constriction of 44 to 49% in cynomolgus macaques and baboons. U46619 and 
      endothelin-1 caused airway constriction in all NHP species, with maximum 
      constrictions of 65 to 91% and 70 to 81%, respectively. In conclusion, PCLS from 
      NHPs represent a valuable ex vivo model for studying bronchoconstriction. All 
      NHPs respond to mediators relevant to human airway disorders such as 
      methacholine, histamine, U46619, and endothelin-1 and are insensitive to the 
      rodent mast cell product serotonin. Only PCLS from cynomolgus macaques and 
      baboons, however, responded also to leukotrienes, suggesting that among all 
      compared species, these two NHPs resemble the human airway mechanisms best.
FAU - Seehase, S
AU  - Seehase S
AD  - Department of Airway Immunology, Fraunhofer Institute for Toxicology and 
      Experimental Medicine, Hannover, Germany.
FAU - Schleputz, M
AU  - Schleputz M
FAU - Switalla, S
AU  - Switalla S
FAU - Matz-Rensing, K
AU  - Matz-Rensing K
FAU - Kaup, F J
AU  - Kaup FJ
FAU - Zoller, M
AU  - Zoller M
FAU - Schlumbohm, C
AU  - Schlumbohm C
FAU - Fuchs, E
AU  - Fuchs E
FAU - Lauenstein, H-D
AU  - Lauenstein HD
FAU - Winkler, C
AU  - Winkler C
FAU - Kuehl, A R
AU  - Kuehl AR
FAU - Uhlig, S
AU  - Uhlig S
FAU - Braun, A
AU  - Braun A
FAU - Sewald, K
AU  - Sewald K
FAU - Martin, C
AU  - Martin C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20110623
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Bronchoconstrictor Agents)
SB  - IM
MH  - Animals
MH  - *Bronchoconstriction/drug effects
MH  - Bronchoconstrictor Agents/pharmacology
MH  - Callithrix
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - Lung/drug effects/*physiology
MH  - Macaca fascicularis
MH  - Macaca mulatta
MH  - Male
MH  - Mice
MH  - Microscopy, Video
MH  - Papio
MH  - Rats
MH  - Species Specificity
EDAT- 2011/06/28 06:00
MHDA- 2012/01/12 06:00
CRDT- 2011/06/25 06:00
PHST- 2011/06/25 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2012/01/12 06:00 [medline]
AID - japplphysiol.00162.2011 [pii]
AID - 10.1152/japplphysiol.00162.2011 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2011 Sep;111(3):791-8. doi: 
      10.1152/japplphysiol.00162.2011. Epub 2011 Jun 23.