PMID- 21701171
OWN - NLM
STAT- MEDLINE
DCOM- 20111216
LR  - 20220409
IS  - 1421-9751 (Electronic)
IS  - 0008-6312 (Linking)
VI  - 118
IP  - 4
DP  - 2011
TI  - Cardiospecific microRNA plasma levels correlate with troponin and cardiac 
      function in patients with ST elevation myocardial infarction, are selectively 
      dependent on renal elimination, and can be detected in urine samples.
PG  - 217-26
LID - 10.1159/000328869 [doi]
AB  - OBJECTIVES: Circulating microRNAs (miRNAs) are promising as biomarkers for 
      various diseases. We examined the release patterns of cardiospecific miRNAs in a 
      closed-chest, large animal ischemia-reperfusion model and in patients with ST 
      elevation myocardial infarction (STEMI). METHODS: Six anesthetized pigs were 
      subjected to coronary occlusion-reperfusion. Plasma, urine, and clinical 
      parameters were collected from 25 STEMI patients undergoing primary percutaneous 
      coronary intervention. miRNA was extracted and measured with qPCR. RESULTS: In 
      the pig reperfusion model miR-1, miR-133a, and miR-208b increased rapidly in 
      plasma with a peak at 120 min, while miR-499-5p remained elevated longer. In 
      patients with STEMI all 4 miRNAs increased abruptly from 70-fold to 3,000-fold in 
      plasma, with a peak within 12 h (p < 0.01). miR-1 and miR-133a both correlated 
      strongly with the glomerular filtration rate (GFR), indicating renal elimination. 
      This was confirmed by detection of miR-1 and miR-133a, but not miR-208b or 
      miR-499-5p, in urine. Peak values of miR-208b correlated with peak troponin and 
      the ejection fraction. CONCLUSION: We demonstrate a distinct and rapid increase 
      in levels of cardiospecific miRNA in the circulation after myocardial infarction. 
      Release of miRNAs correlated with cardiomyocyte necrosis markers, the ejection 
      fraction, and the GFR, indicating a possible role for these molecules as 
      biomarkers for the diagnosis of STEMI as well as the prediction of long-term 
      complications.
CI  - Copyright (c) 2011 S. Karger AG, Basel.
FAU - Gidlof, Olof
AU  - Gidlof O
AD  - Department of Cardiology, Skane University Hospital, Lund University, Lund, 
      Sweden.
FAU - Andersson, Patrik
AU  - Andersson P
FAU - van der Pals, Jesper
AU  - van der Pals J
FAU - Gotberg, Matthias
AU  - Gotberg M
FAU - Erlinge, David
AU  - Erlinge D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110624
PL  - Switzerland
TA  - Cardiology
JT  - Cardiology
JID - 1266406
RN  - 0 (Biomarkers)
RN  - 0 (MicroRNAs)
RN  - 0 (Troponin T)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Biomarkers/blood/urine
MH  - Female
MH  - Glomerular Filtration Rate
MH  - Humans
MH  - Male
MH  - MicroRNAs/*blood/urine
MH  - Middle Aged
MH  - Myocardial Infarction/*blood/pathology/urine
MH  - Myocytes, Cardiac/pathology
MH  - Necrosis
MH  - ROC Curve
MH  - Stroke Volume
MH  - Swine
MH  - Troponin T/blood
EDAT- 2011/06/28 06:00
MHDA- 2011/12/17 06:00
CRDT- 2011/06/25 06:00
PHST- 2010/11/30 00:00 [received]
PHST- 2011/04/27 00:00 [accepted]
PHST- 2011/06/25 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2011/12/17 06:00 [medline]
AID - 000328869 [pii]
AID - 10.1159/000328869 [doi]
PST - ppublish
SO  - Cardiology. 2011;118(4):217-26. doi: 10.1159/000328869. Epub 2011 Jun 24.