PMID- 21703032
OWN - NLM
STAT- MEDLINE
DCOM- 20111019
LR  - 20220316
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 8
DP  - 2011 Jun 27
TI  - The assessment of efficacy of porcine reproductive respiratory syndrome virus 
      inactivated vaccine based on the viral quantity and inactivation methods.
PG  - 323
LID - 10.1186/1743-422X-8-323 [doi]
AB  - BACKGROUND: There have been many efforts to develop efficient vaccines for the 
      control of porcine reproductive and respiratory syndrome virus (PRRSV). Although 
      inactivated PRRSV vaccines are preferred for their safety, they are weak at 
      inducing humoral immune responses and controlling field PRRSV infection, 
      especially when heterologous viruses are involved. RESULTS: In all groups, the 
      sample to positive (S/P) ratio of IDEXX ELISA and the virus neutralization (VN) 
      titer remained negative until challenge. While viremia did not reduce in the 
      vaccinated groups, the IDEXX-ELISA-specific immunoglobulin G increased more 
      rapidly and to significantly greater levels 7 days after the challenge in all the 
      vaccinated groups compared to the non-vaccinated groups (p < 0.05). VN titer was 
      significantly different in the 106 PFU/mL PRRSV vaccine-inoculated and binary 
      ethylenimine (BEI)-inactivated groups 22 days after challenge (p < 0.05). 
      Consequently, the inactivated vaccines tested in this study provided weak memory 
      responses with sequential challenge without any obvious active immune responses 
      in the vaccinated pigs. CONCLUSIONS: The inactivated vaccine failed to show the 
      humoral immunity, but it showed different immune response after the challenge 
      compared to mock group. Although the 106 PFU/mL-vaccinated and BEI-inactivated 
      groups showed significantly greater VN titers 22 days after challenge, all the 
      groups were already negative for viremia.
FAU - Kim, Hyunil
AU  - Kim H
AD  - Optifarm Solution Inc., 48 Songnam-ri, Seonggeo-eup, Cheonan, Korea.
FAU - Kim, Hye Kwon
AU  - Kim HK
FAU - Jung, Jung Ho
AU  - Jung JH
FAU - Choi, Yoo Jung
AU  - Choi YJ
FAU - Kim, Jiho
AU  - Kim J
FAU - Um, Chang Gyu
AU  - Um CG
FAU - Hyun, Su Bin
AU  - Hyun SB
FAU - Shin, Sungho
AU  - Shin S
FAU - Lee, Byeongchun
AU  - Lee B
FAU - Jang, Goo
AU  - Jang G
FAU - Kang, Bo Kyu
AU  - Kang BK
FAU - Moon, Hyoung Joon
AU  - Moon HJ
FAU - Song, Dae Sub
AU  - Song DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110627
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoglobulin G/blood
MH  - Immunologic Memory
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Vaccines, Inactivated/administration & dosage/immunology
MH  - Viral Load
MH  - Viral Vaccines/administration & dosage/*immunology
MH  - Viremia/prevention & control
PMC - PMC3141684
EDAT- 2011/06/28 06:00
MHDA- 2011/10/20 06:00
PMCR- 2011/06/27
CRDT- 2011/06/28 06:00
PHST- 2011/02/22 00:00 [received]
PHST- 2011/06/27 00:00 [accepted]
PHST- 2011/06/28 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2011/10/20 06:00 [medline]
PHST- 2011/06/27 00:00 [pmc-release]
AID - 1743-422X-8-323 [pii]
AID - 10.1186/1743-422X-8-323 [doi]
PST - epublish
SO  - Virol J. 2011 Jun 27;8:323. doi: 10.1186/1743-422X-8-323.