PMID- 21703666
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR  - 20151119
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 114
IP  - 3
DP  - 2012 May
TI  - Effects of genistein on gonadotropic cells in immature female rats.
PG  - 270-5
LID - 10.1016/j.acthis.2011.06.003 [doi]
AB  - The effects of genistein on pituitary gonadotropic cells of immature female rats 
      were examined and compared to actions of the synthetic estrogen, 
      17alpha-ethynylestradiol. Immature female rats received 50mg/kg/bw of genistein in 
      dimethylsulfoxide (DMSO) subcutaneously (s.c.) daily for 3 days at 18, 19 and 20 
      days of age. A second group was injected with 1mug/kg of 17alpha-ethynylestradiol in 
      olive oil in the same schedule. The genistein control group received DMSO only, 
      while 17alpha-ethynylestradiol controls were given sterile olive oil only. Changes in 
      cell number per mm(2), cell volume and volume density of follicle-stimulating 
      (FSH) and luteinizing (LH) immunolabeled cells were evaluated by morphometry and 
      stereology. Genistein induced significant increases in the number of FSH cells 
      (by 21%) and LH cells (by 20%) per mm(2) compared to corresponding controls. 
      Volumes of FSH and LH cells were significantly increased by 19.7% and 20% and 
      their volume densities by 20% and 20.2%, respectively. Estradiol markedly 
      affected gonadotropes in the same manner, but to a greater extent. It can be 
      concluded that genistein acted as an estrogenic agonist in the pituitaries of 
      immature female rats, and as such, stimulated gonadotropic cells.
CI  - Copyright A(c) 2011 Elsevier GmbH. All rights reserved.
FAU - Medigovic, Ivana
AU  - Medigovic I
AD  - Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 
      Serbia. ivana84@ibiss.bg.ac.rs
FAU - Manojlovic-Stojanoski, Milica
AU  - Manojlovic-Stojanoski M
FAU - Trifunovic, Svetlana
AU  - Trifunovic S
FAU - Ristic, Natasa
AU  - Ristic N
FAU - Milosevic, Verica
AU  - Milosevic V
FAU - Zikic, Dragan
AU  - Zikic D
FAU - Nestorovic, Natasa
AU  - Nestorovic N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110623
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Olive Oil)
RN  - 0 (Plant Oils)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - DH2M523P0H (Genistein)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Count
MH  - Dimethyl Sulfoxide
MH  - Estradiol/analogs & derivatives/pharmacology
MH  - Female
MH  - Follicle Stimulating Hormone/*agonists/biosynthesis
MH  - Genistein/*pharmacology
MH  - Gonadotrophs/cytology/*drug effects/physiology
MH  - Immunohistochemistry
MH  - Injections, Subcutaneous
MH  - Luteinizing Hormone/*agonists/biosynthesis
MH  - Microscopy
MH  - Olive Oil
MH  - Plant Oils
MH  - Rats
MH  - Rats, Wistar
EDAT- 2011/06/28 06:00
MHDA- 2012/06/23 06:00
CRDT- 2011/06/28 06:00
PHST- 2011/03/30 00:00 [received]
PHST- 2011/05/25 00:00 [revised]
PHST- 2011/05/30 00:00 [accepted]
PHST- 2011/06/28 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
AID - S0065-1281(11)00093-6 [pii]
AID - 10.1016/j.acthis.2011.06.003 [doi]
PST - ppublish
SO  - Acta Histochem. 2012 May;114(3):270-5. doi: 10.1016/j.acthis.2011.06.003. Epub 
      2011 Jun 23.