PMID- 21704001
OWN - NLM
STAT- MEDLINE
DCOM- 20120120
LR  - 20230603
IS  - 1528-0012 (Electronic)
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 141
IP  - 5
DP  - 2011 Nov
TI  - Spontaneous, immune-mediated gastric inflammation in SAMP1/YitFc mice, a model of 
      Crohn's-like gastritis.
PG  - 1709-19
LID - 10.1053/j.gastro.2011.06.041 [doi]
AB  - BACKGROUND & AIMS: Crohn's disease (CD) can develop in any region of the 
      gastrointestinal tract, including the stomach. The etiology and pathogenesis of 
      Crohn's gastritis are poorly understood, treatment approaches are limited, and 
      there are not many suitable animal models for study. We characterized the 
      features and mechanisms of chronic gastritis in SAMP1/YitFc (SAMP) mice, a 
      spontaneous model of CD-like ileitis, along with possible therapeutic approaches. 
      METHODS: Stomachs from specific pathogen-free and germ-free SAMP and AKR mice 
      (controls) were evaluated histologically; the presence of Helicobacter spp was 
      tested in fecal pellets by polymerase chain reaction analysis. In vivo gastric 
      permeability was quantified by fractional excretion of sucrose, and epithelial 
      tight junction protein expression was measured by quantitative 
      reverse-transcription polymerase chain reaction analysis. The effects of a proton 
      pump inhibitor (PPI) or corticosteroids were measured, and the ability of 
      pathogenic immune cells to mediate gastritis was assessed in adoptive transfer 
      experiments. RESULTS: SAMP mice developed Helicobacter-negative gastritis, 
      characterized by aggregates of mononuclear cells, diffuse accumulation of 
      neutrophils, and disruption of epithelial architecture; SAMP mice also had 
      increased gastric permeability compared with controls, without alterations in 
      expression of tight junction proteins. The gastritis and associated permeability 
      defect observed in SAMP mice were independent of bacterial colonization and 
      reduced by administration of corticosteroids but not a PPI. CD4(+) T cells 
      isolated from draining mesenteric lymph nodes of SAMP mice were sufficient to 
      induce gastritis in recipient SCID mice. CONCLUSIONS: In SAMP mice, gastritis 
      develops spontaneously and has many features of CD-like ileitis. These mice are a 
      useful model to study Helicobacter-negative, immune-mediated Crohn's gastritis.
CI  - Copyright (c) 2011 AGA Institute. Published by Elsevier Inc. All rights reserved.
FAU - Reuter, Brian K
AU  - Reuter BK
AD  - Centre of Excellence for Gastrointestinal Inflammation and Immunity Research, 
      University of Alberta, Edmonton, Alberta, Canada.
FAU - Pastorelli, Luca
AU  - Pastorelli L
FAU - Brogi, Marco
AU  - Brogi M
FAU - Garg, Rekha R
AU  - Garg RR
FAU - McBride, James A
AU  - McBride JA
FAU - Rowlett, Robert M
AU  - Rowlett RM
FAU - Arrieta, Marie C
AU  - Arrieta MC
FAU - Wang, Xiao-Ming
AU  - Wang XM
FAU - Keller, Erik J
AU  - Keller EJ
FAU - Feldman, Sanford H
AU  - Feldman SH
FAU - Mize, James R
AU  - Mize JR
FAU - Cominelli, Fabio
AU  - Cominelli F
FAU - Meddings, Jonathan B
AU  - Meddings JB
FAU - Pizarro, Theresa T
AU  - Pizarro TT
LA  - eng
GR  - R01 DK056762-09/DK/NIDDK NIH HHS/United States
GR  - DK056762/DK/NIDDK NIH HHS/United States
GR  - DK055812/DK/NIDDK NIH HHS/United States
GR  - P01 DK091222/DK/NIDDK NIH HHS/United States
GR  - R01 DK055812-13/DK/NIDDK NIH HHS/United States
GR  - R01 DK056762/DK/NIDDK NIH HHS/United States
GR  - P01 DK057880/DK/NIDDK NIH HHS/United States
GR  - P01 DK057880-08/DK/NIDDK NIH HHS/United States
GR  - DK057880/DK/NIDDK NIH HHS/United States
GR  - P01 DK091222-01/DK/NIDDK NIH HHS/United States
GR  - R56 DK055812/DK/NIDDK NIH HHS/United States
GR  - R01 DK055812/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110623
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Proton Pump Inhibitors)
SB  - IM
MH  - Adrenal Cortex Hormones/therapeutic use
MH  - Animals
MH  - Crohn Disease/drug therapy/*immunology/*physiopathology
MH  - Disease Models, Animal
MH  - Feces/microbiology
MH  - Gastritis/drug therapy/*immunology/*physiopathology
MH  - Helicobacter/isolation & purification
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Mice, Mutant Strains
MH  - Mice, SCID
MH  - Proton Pump Inhibitors/therapeutic use
MH  - Tight Junctions/physiology
MH  - Treatment Outcome
PMC - PMC3197754
MID - NIHMS307665
EDAT- 2011/06/28 06:00
MHDA- 2012/01/21 06:00
PMCR- 2012/11/01
CRDT- 2011/06/28 06:00
PHST- 2011/02/10 00:00 [received]
PHST- 2011/05/27 00:00 [revised]
PHST- 2011/06/07 00:00 [accepted]
PHST- 2011/06/28 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2012/01/21 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - S0016-5085(11)00820-1 [pii]
AID - 10.1053/j.gastro.2011.06.041 [doi]
PST - ppublish
SO  - Gastroenterology. 2011 Nov;141(5):1709-19. doi: 10.1053/j.gastro.2011.06.041. 
      Epub 2011 Jun 23.