PMID- 21704607
OWN - NLM
STAT- MEDLINE
DCOM- 20120809
LR  - 20171116
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 412
IP  - 19-20
DP  - 2011 Sep 18
TI  - Persistent elevation of paraoxonase-1 specific enzyme activity after weight 
      reduction in obese non-diabetic men with metabolic syndrome.
PG  - 1835-41
LID - 10.1016/j.cca.2011.06.018 [doi]
AB  - BACKGROUND: Paraoxonase-1 (PON1) is an esterase associated with the high-density 
      lipoprotein (HDL) in serum. To date, there have been few reports about 
      circulating PON1 protein concentration and specific activity in subjects with 
      metabolic syndrome (MetS). More importantly, it is unknown whether weight loss 
      could alter PON1 protein expression or specific activity in obese non-diabetic 
      men with MetS. METHODS: We prospectively enrolled a total of 40 obese 
      non-diabetic men with MetS. Among them, 22 subjects finished the 3-month course 
      of weight loss program and complied for longer follow-ups post-weight loss at the 
      3rd, 12th, and 18th month from the beginning of the program. Twenty-six healthy 
      volunteers served as controls. Serum circulating PON1 concentration was measured 
      by an enzyme linked immunosorbent kit (ELISA) and PON1 activity was measured by 
      an automated PON1 activity assay. RESULTS: Obese non-diabetic men with MetS 
      (n=40) had a higher PON1 protein concentration (31.0 +/- 11.3 vs. 24.8 +/- 9.7 mug/ml, 
      p=0.025) but lower specific enzyme activity (7.5 +/- 4.0 vs. 11.2 +/- 7.2 mU/mug, 
      p=0.023) than those of the controls. Multivariate regression analysis of baseline 
      PON1 specific activity revealed that adiponectin was a significant positive 
      predictor (p=0.044) while monocyte chemotactic protein-1 (MCP-1) was a negative 
      predictor (p=0.031). After a 3-month weight loss program, obese MetS men (n=22) 
      had a significant weight reduction (95.8 +/- 9.0 to 86.3 +/- 10.4 kg, with a 9.9 +/- 
      5.4% decrease, p<0.001). PON1 protein decreased significantly after weight loss 
      and kept declining through the 3rd month till the 18th month follow-up. PON1 
      specific enzyme activity (baseline 7.5 +/- 2.6 mU/mug) increased significantly after 
      weight loss and kept increasing through the 12th month till the 18th month 
      follow-ups (11.8 +/- 6.4 mU/mug, p=0.001 vs. baseline). CONCLUSIONS: Weight loss by 
      a 3-month diet and exercise program time-sequentially increased PON1 specific 
      enzyme activity in obese non-diabetic men with MetS.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Liang, Kae-Woei
AU  - Liang KW
AD  - Cardiovascular Center, Taichung Veterans General Hospital, Taiwan.
FAU - Lee, Wen-Jane
AU  - Lee WJ
FAU - Lee, I-Te
AU  - Lee IT
FAU - Lee, Wen-Lieng
AU  - Lee WL
FAU - Lin, Shih-Yi
AU  - Lin SY
FAU - Hsu, Shih-Lan
AU  - Hsu SL
FAU - Wan, Chu-Jen
AU  - Wan CJ
FAU - Yu, Chen-Yuan
AU  - Yu CY
FAU - Tsai, I-Chen
AU  - Tsai IC
FAU - Fu, Chia-Po
AU  - Fu CP
FAU - Ting, Chih-Tai
AU  - Ting CT
FAU - Sheu, Wayne H-H
AU  - Sheu WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110617
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - EC 3.1.8.1 (Aryldialkylphosphatase)
SB  - IM
MH  - Adult
MH  - Aryldialkylphosphatase/*metabolism
MH  - Humans
MH  - Male
MH  - Metabolic Syndrome/complications/*enzymology/physiopathology
MH  - Middle Aged
MH  - Obesity/complications/*enzymology/physiopathology
MH  - Prospective Studies
MH  - *Weight Loss
EDAT- 2011/06/28 06:00
MHDA- 2012/08/10 06:00
CRDT- 2011/06/28 06:00
PHST- 2011/01/13 00:00 [received]
PHST- 2011/06/13 00:00 [revised]
PHST- 2011/06/13 00:00 [accepted]
PHST- 2011/06/28 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2012/08/10 06:00 [medline]
AID - S0009-8981(11)00351-2 [pii]
AID - 10.1016/j.cca.2011.06.018 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2011 Sep 18;412(19-20):1835-41. doi: 10.1016/j.cca.2011.06.018. 
      Epub 2011 Jun 17.