PMID- 2170538
OWN - NLM
STAT- MEDLINE
DCOM- 19901106
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 95
IP  - 4
DP  - 1990 Oct
TI  - Retroviral expression of transforming growth factor-alpha does not transform 
      fibroblasts or keratinocytes.
PG  - 382-7
AB  - Transforming growth factor alpha (TGF alpha) is a peptide so named because it 
      helps to impart anchorage-independent growth to normal rat kidney (NRK) cells in 
      vitro and is secreted by many rodent and human tumor cells. To directly 
      investigate the transforming properties of this factor, we constructed a 
      replication-defective murine retrovirus that expresses the human sequence coding 
      for TGF alpha. Infection of NIH/3T3 cells with the TGF alpha retrovirus led to 
      the integration of a transcriptionally active provirus and overexpression of 
      biologically active TGF alpha, but failed to induce morphologic transformation. 
      Similarly, the TGF alpha retrovirus failed to induce morphologic transformation 
      of five other types of rodent fibroblasts. We also investigated the effect of TGF 
      alpha expression on the growth of BALB/MK mouse keratinocytes, which require 
      epidermal growth factor (EGF) for proliferation. We show that exogenously added 
      TGF alpha is an extremely potent mitogen for BALB/MK cells. However, retroviral 
      expression of TGF alpha in BALB/MK cells failed to relieve dependence on 
      exogenously added EGF (or TGF alpha) for cell growth. These results suggest that 
      overexpression of TGF alpha does not, by itself, transform rodent fibroblasts or 
      keratinocytes.
FAU - Finzi, E
AU  - Finzi E
AD  - Laboratory of Cellular and Molecular Biology, National Cancer Institute, 
      Bethesda, Maryland.
FAU - Fleming, T
AU  - Fleming T
FAU - Pierce, J H
AU  - Pierce JH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Cell Transformation, Neoplastic
MH  - DNA Replication
MH  - DNA Transposable Elements
MH  - Fibroblasts/cytology
MH  - Humans
MH  - Keratinocytes/*cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Moloney murine leukemia virus/*genetics
MH  - Proviruses/genetics
MH  - Restriction Mapping
MH  - Transcription, Genetic
MH  - *Transfection
MH  - Transforming Growth Factor alpha/biosynthesis/*genetics/physiology
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - S0022-202X(90)90213-P [pii]
AID - 10.1111/1523-1747.ep12555464 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1990 Oct;95(4):382-7. doi: 10.1111/1523-1747.ep12555464.