PMID- 21706349
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20211020
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 4
DP  - 2012 Apr
TI  - Characterization, and expression profile of a phenylalanine ammonia lyase gene 
      from Jatropha curcas L.
PG  - 3443-52
LID - 10.1007/s11033-011-1116-4 [doi]
AB  - A PAL gene designated as JcPAL1 was cloned from J. curcas L. The full-length is 
      2336 bp in size with one intron and two exons, encoding a polypeptide of 713 
      amino acids. Its 5'-upstream region is rich in putative cis-elements including 
      not only PAL typical TATA box, L-box and transcriptional initiation site (TIS) 
      but also light responding motifs. Expression pattern analysis indicated that 
      JcPAL1 were expressed in all tissues, most highly in flowers. When Treated with 
      ABA, GA3, high and low temperature, expression of JcPAL1 were induced. 
      Recombinant JcPAL1 has a pH optimum at 8.7 and a temperature optimum at 60 degrees C in 
      100 mM Tris-HCl buffer. The Km and Kcat values are 0.125 mM and 1.73 S(-1) for L: 
      -phenylalanine, and 1.312 mM and 0.109 S(-1) for L: -tyrosine, respectively. 
      These findings suggested that JcPAL1 might involve in the J. curcas responding to 
      various stresses and L: -Phe should be its true physiological substrate. This 
      study is essential prior to uncover whether and how the PAL initiated 
      phenylpropanoid metabolic networks functioning in the defense responses of J. 
      curcas.
FAU - Gao, Jihai
AU  - Gao J
AD  - School of Life Sciences, Sichuan University, 24 South Section 1, 1st Ring Road, 
      Chengdu, 610065, People's Republic of China.
FAU - Zhang, Shuwen
AU  - Zhang S
FAU - Cai, Feng
AU  - Cai F
FAU - Zheng, Xiaojiang
AU  - Zheng X
FAU - Lin, Na
AU  - Lin N
FAU - Qin, Xiaobo
AU  - Qin X
FAU - Ou, Yangchao
AU  - Ou Y
FAU - Gu, Xiaoping
AU  - Gu X
FAU - Zhu, Xihong
AU  - Zhu X
FAU - Xu, Ying
AU  - Xu Y
FAU - Chen, Fang
AU  - Chen F
LA  - eng
SI  - GENBANK/DQ883805
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110626
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (DNA, Complementary)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
SB  - IM
MH  - Base Sequence
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Plant/*genetics
MH  - Jatropha/*enzymology/*genetics
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Phenylalanine Ammonia-Lyase/*genetics/metabolism
MH  - Plant Proteins/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Recombinant Proteins/metabolism
MH  - Regulatory Sequences, Nucleic Acid/genetics
MH  - Sequence Analysis, DNA
MH  - Transcriptome/genetics
EDAT- 2011/06/28 06:00
MHDA- 2012/06/29 06:00
CRDT- 2011/06/28 06:00
PHST- 2011/01/05 00:00 [received]
PHST- 2011/06/20 00:00 [accepted]
PHST- 2011/06/28 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
AID - 10.1007/s11033-011-1116-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Apr;39(4):3443-52. doi: 10.1007/s11033-011-1116-4. Epub 2011 
      Jun 26.