PMID- 21708140
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20220318
IS  - 1872-7786 (Electronic)
IS  - 0009-2797 (Linking)
VI  - 193
IP  - 2
DP  - 2011 Sep 5
TI  - Effect of galangin supplementation on oxidative damage and inflammatory changes 
      in fructose-fed rat liver.
PG  - 141-8
LID - 10.1016/j.cbi.2011.06.003 [doi]
AB  - The study examined the effects of galangin (GA) on oxidative stress, inflammatory 
      cytokine levels and nuclear factor-kappa B (NF-kappaB) activation in fructose-fed rat 
      liver. Adult male albino Wistar rats were divided into 4 groups. Groups 1 and 4 
      received the control diet containing starch as the source of carbohydrate while 
      groups 2 and 3 were fed a diet containing fructose. Groups 3 and 4 additionally 
      received GA (100mug/kg, p.o) from the 15th day. At the end of 60 days, the levels 
      of plasma glucose, insulin and triglycerides, insulin sensitivity indices and 
      oxidative stress markers in the liver were determined. Cytokines of interest were 
      assayed by ELISA and RT-PCR and NF-kappaB p65 nuclear translocation by Western blot 
      and RT-PCR. Compared to control diet-fed animals, fructose-fed animals developed 
      hyperglycemia, hyperinsulinemia, hypertriglyceridemia and insulin resistance (IR) 
      (all p<0.01). GA prevented the rise in plasma glucose, insulin and triglycerides 
      and improved insulin sensitivity. Tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-6 (IL-6) levels in plasma and the mRNA and protein levels of TNF-alpha 
      and transforming growth factor-beta1(TGF-beta(1)) in liver were significantly higher in 
      fructose-fed rats than control rats. However, treatment with GA downregulated the 
      expression of these cytokines. Translocation of NF-kappaB into the nucleus was also 
      increased in fructose diet-fed animals, which was prevented by GA. These results 
      suggest that GA prevents oxidative damage and has a downregulatory effect on the 
      inflammatory pathway in liver of fructose-fed rats.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Sivakumar, Allur Subramaniyan
AU  - Sivakumar AS
AD  - Department of Biochemistry and Biotechnology, Annamalai University, 
      Annamalainagar, Tamil Nadu, India.
FAU - Anuradha, Carani Venkatraman
AU  - Anuradha CV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110625
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Antioxidants)
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Flavonoids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Insulin)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipid Peroxides)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 142FWE6ECS (galangin)
RN  - 30237-26-4 (Fructose)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Blood Glucose/drug effects/metabolism
MH  - Body Weight/drug effects
MH  - Cell Nucleus/metabolism
MH  - Chemical and Drug Induced Liver Injury/blood/metabolism/pathology/*prevention & 
      control
MH  - Collagen/metabolism
MH  - Cytosol/metabolism
MH  - Dietary Carbohydrates/administration & dosage/*pharmacology
MH  - *Dietary Supplements
MH  - Flavonoids/administration & dosage/*pharmacology/therapeutic use
MH  - Free Radical Scavengers/administration & dosage/pharmacology
MH  - Fructose/administration & dosage/*pharmacology
MH  - Gene Expression/drug effects/genetics
MH  - Insulin/blood
MH  - Insulin Resistance/physiology
MH  - Interleukin-6/blood
MH  - Lipid Peroxides/blood
MH  - Liver/*drug effects/metabolism/pathology
MH  - Male
MH  - Oxidative Stress/*drug effects
MH  - Protein Carbonylation/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription Factor RelA/genetics/metabolism
MH  - Transforming Growth Factor beta1/genetics
MH  - Triglycerides/blood
MH  - Tumor Necrosis Factor-alpha/blood/genetics
EDAT- 2011/06/29 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/06/29 06:00
PHST- 2011/04/15 00:00 [received]
PHST- 2011/05/27 00:00 [revised]
PHST- 2011/06/07 00:00 [accepted]
PHST- 2011/06/29 06:00 [entrez]
PHST- 2011/06/29 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - S0009-2797(11)00223-7 [pii]
AID - 10.1016/j.cbi.2011.06.003 [doi]
PST - ppublish
SO  - Chem Biol Interact. 2011 Sep 5;193(2):141-8. doi: 10.1016/j.cbi.2011.06.003. Epub 
      2011 Jun 25.