PMID- 21708204
OWN - NLM
STAT- MEDLINE
DCOM- 20120111
LR  - 20171116
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 72
IP  - 10
DP  - 2011 Oct
TI  - Changes in cytokine production and composition of peripheral blood leukocytes 
      during pregnancy are not associated with a difference in the proliferative immune 
      response to the fetus.
PG  - 805-11
LID - 10.1016/j.humimm.2011.05.027 [doi]
AB  - We analyzed peripheral blood from women at term pregnancy for leukocyte 
      composition, in vitro proliferative responses and cytokine production after 
      nonspecific and fetus-specific stimulation. Maternal peripheral blood mononuclear 
      cells (PBMCs) were collected and stimulated with umbilical cord blood (UCB) of 
      the mother's own child, third-party UCB, nonspecific stimulus phytohemagglutinin, 
      and anti-CD3 antibody, with PBMCs of nonpregnant women (cPBMC) as controls. Nine 
      combinations of patient, child, third party child, and controls were selected on 
      basis of sharing one human leukocyte antigen (HLA)-DR antigen. The response of 
      mPBMC upon specific stimulation with fetal antigens was similar to that of cPBMC. 
      No differences were found when comparing the mother's response upon stimulation 
      to her own child with stimulation to that with a control child. Nonspecific 
      stimulation with phytohemagglutinin and anti-CD3 antibody did not reveal a 
      difference in proliferation rate between mPBMC and cPBMC. However, mPBMC 
      contained a higher percentage of CD14(+) cells (p = 0.001) and activated T cells 
      (CD25(dim), p < 0.0001), but a lower percentage CD16(-)CD56(bright) natural 
      killer (NK) cells (p = 0.001) and CD16(+)CD56(+) NK cells (p = 0.003). mPBMC 
      produced more interleukin (IL)-6, IL-10, and IL-17 compared with cPBMC (p < 
      0.05). We found differences in lymphocyte composition and cytokine production 
      between mPBMC and cPBMC. These differences did not result in quantitative changes 
      in proliferative responses during pregnancy compared with responses in 
      nonpregnant controls.
CI  - Copyright (c) 2011 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Lashley, Lisa E E L O
AU  - Lashley LE
AD  - Department of Obstetrics, Leiden University Medical Center, Leiden, the 
      Netherlands.
FAU - van der Hoorn, Marie-Louise P
AU  - van der Hoorn ML
FAU - van der Mast, Barbara J
AU  - van der Mast BJ
FAU - Tilburgs, Tamara
AU  - Tilburgs T
FAU - van der Lee, Nadine
AU  - van der Lee N
FAU - van der Keur, Carin
AU  - van der Keur C
FAU - van Beelen, Els
AU  - van Beelen E
FAU - Roelen, Dave L
AU  - Roelen DL
FAU - Claas, Frans H J
AU  - Claas FH
FAU - Scherjon, Sicco A
AU  - Scherjon SA
LA  - eng
PT  - Journal Article
DEP - 20110612
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies)
RN  - 0 (CD3 Complex)
RN  - 0 (CD56 Antigen)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Receptors, IgG)
RN  - 10028-17-8 (Tritium)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Adult
MH  - Antibodies/immunology/*pharmacology
MH  - CD3 Complex/immunology
MH  - CD56 Antigen/analysis/immunology
MH  - Case-Control Studies
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis/immunology
MH  - Cytotoxicity, Immunologic
MH  - Female
MH  - Fetal Blood/*immunology
MH  - Fetus
MH  - Flow Cytometry
MH  - HLA-DR Antigens/analysis/immunology
MH  - Humans
MH  - Immunoassay
MH  - *Leukocytes, Mononuclear/cytology/drug effects/immunology/metabolism
MH  - Lymphocyte Activation/drug effects
MH  - Phytohemagglutinins/immunology/*pharmacology
MH  - Pregnancy
MH  - Receptors, IgG/analysis/immunology
MH  - Thymidine/metabolism
MH  - Tritium/metabolism
EDAT- 2011/06/29 06:00
MHDA- 2012/01/12 06:00
CRDT- 2011/06/29 06:00
PHST- 2011/03/17 00:00 [received]
PHST- 2011/05/24 00:00 [revised]
PHST- 2011/05/25 00:00 [accepted]
PHST- 2011/06/29 06:00 [entrez]
PHST- 2011/06/29 06:00 [pubmed]
PHST- 2012/01/12 06:00 [medline]
AID - S0198-8859(11)00143-1 [pii]
AID - 10.1016/j.humimm.2011.05.027 [doi]
PST - ppublish
SO  - Hum Immunol. 2011 Oct;72(10):805-11. doi: 10.1016/j.humimm.2011.05.027. Epub 2011 
      Jun 12.