PMID- 21708216
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20131121
IS  - 1879-3185 (Electronic)
IS  - 0300-483X (Linking)
VI  - 287
IP  - 1-3
DP  - 2011 Sep 5
TI  - Negative regulation of NaF-induced apoptosis by Bad-CAII complex.
PG  - 131-6
LID - 10.1016/j.tox.2011.06.007 [doi]
AB  - Fluoride is used to prevent caries in dentistry. However, its mechanism of 
      cytotoxicity induction is unclear. This study was undertaken to determine whether 
      sodium fluoride (NaF) induces apoptosis in human oral cells and if so, whether 
      Bad protein is involved in the process. NaF showed higher cytotoxicity and 
      apoptosis-inducing activity against human oral squamous cell carcinoma cells 
      (HSC-2) than against human gingival fibroblasts (HGF). Western blot analysis 
      showed that NaF enhanced the expression and dephosphorylation of Bad protein. 
      This study demonstrates for the first time that Bad protein forms a complex with 
      carbonic anhydrase II (CAII), and NaF stimulates the detachment of CAII from the 
      Bad-CAII complex and the replacement by the formation of Bad-Bcl-2 complex. 
      Knockdown of Bad and CAII mRNA by siRNA inhibited and enhanced the NaF-induced 
      caspase activation, respectively. The present study suggests that CAII negatively 
      regulates the NaF-induced apoptosis by forming a complex with Bad.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Otsuki, S
AU  - Otsuki S
AD  - Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama 
      350-0283, Japan.
FAU - Sugiyama, K
AU  - Sugiyama K
FAU - Amano, O
AU  - Amano O
FAU - Yasui, T
AU  - Yasui T
FAU - Sakagami, H
AU  - Sakagami H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110617
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (BAD protein, human)
RN  - 0 (bcl-Associated Death Protein)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 7)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Carbonic Anhydrase II/*metabolism
MH  - Caspase 3/metabolism
MH  - Caspase 7/metabolism
MH  - Cell Line, Tumor
MH  - Humans
MH  - Sodium Fluoride/*toxicity
MH  - bcl-Associated Death Protein/*metabolism
EDAT- 2011/06/29 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/06/29 06:00
PHST- 2011/05/09 00:00 [received]
PHST- 2011/06/08 00:00 [revised]
PHST- 2011/06/10 00:00 [accepted]
PHST- 2011/06/29 06:00 [entrez]
PHST- 2011/06/29 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - S0300-483X(11)00233-2 [pii]
AID - 10.1016/j.tox.2011.06.007 [doi]
PST - ppublish
SO  - Toxicology. 2011 Sep 5;287(1-3):131-6. doi: 10.1016/j.tox.2011.06.007. Epub 2011 
      Jun 17.