PMID- 21708226
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20171116
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1810
IP  - 9
DP  - 2011 Sep
TI  - Monocyte-endothelium-smooth muscle cell interaction in co-culture: proliferation 
      and cytokine productions in response to advanced glycation end products.
PG  - 907-12
LID - 10.1016/j.bbagen.2011.06.005 [doi]
AB  - BACKGROUND: During hyperglycemia, reducing sugars react with the amino groups to 
      form Amadori products which then form advanced glycation end-products (AGEs). 
      Studies have shown that the AGEs and the receptor binding generated reactive 
      oxygen species, and triggered secretion of cytokines contributing to the local 
      regulations of proliferation and inflammation in cells. Interaction of vessel 
      wall cells and monocytes may trigger the processes leading to atherosclerosis. We 
      evaluated the effects of AGEs on smooth muscle cell (SMC) proliferation and 
      cytokine synthesis in co-cultures of human monocytes (THP-1), endothelial cells 
      (HUVEC) and aortic vascular smooth muscle cell (SMC) to clarify the effects of 
      AGEs on vascular cells and to investigate the mechanisms of arteriosclerosis. 
      METHODS: Glycolaldehyde-induced AGEs (glycol-AGEs) was prepared. The THP-1 and 
      HUVEC were cultured with SMC in transwell plates with 100 mug/ml of glycol-AGEs 
      for 24 to 48 h. RESULTS: The proliferation of SMC was induced by glycol-AGEs in 
      the co-culture system. Moreover, SMC treated with glycol-AGEs also expressed 
      interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1), and the 
      level of cytokines expression was significantly elevated in the co-culture system 
      of HUVEC and THP-1 when treated with glycol-AGEs. CONCLUSION: These results 
      suggest that employing a co-culture system is necessary to investigate the 
      synergistic effects of AGEs on intercellular cellular interactions and it creates 
      a more in vivo-like environment for AGEs implicated atherosclerosis research. 
      GENERAL SIGNIFICANCE: All three cell types are required to be investigated 
      together to understand the effects of AGEs on intercellular interactions 
      occurring among these cells.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Nam, Mi-Hyun
AU  - Nam MH
AD  - Division of Food Bioscience and Technology, College of Life Science & 
      Biotechnology, Korea University, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic 
      of Korea.
FAU - Lee, Hyun-Sun
AU  - Lee HS
FAU - Seomun, Young
AU  - Seomun Y
FAU - Lee, Yanhouy
AU  - Lee Y
FAU - Lee, Kwang-Won
AU  - Lee KW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110625
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aorta/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Coculture Techniques
MH  - Endothelium, Vascular/*metabolism
MH  - Glycation End Products, Advanced/*pharmacology
MH  - Humans
MH  - Interleukin-6/*biosynthesis
MH  - Monocytes/*metabolism
MH  - Muscle, Smooth, Vascular/metabolism
MH  - Myocytes, Smooth Muscle/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2011/06/29 06:00
MHDA- 2011/09/20 06:00
CRDT- 2011/06/29 06:00
PHST- 2010/12/11 00:00 [received]
PHST- 2011/06/08 00:00 [revised]
PHST- 2011/06/09 00:00 [accepted]
PHST- 2011/06/29 06:00 [entrez]
PHST- 2011/06/29 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
AID - S0304-4165(11)00131-0 [pii]
AID - 10.1016/j.bbagen.2011.06.005 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2011 Sep;1810(9):907-12. doi: 10.1016/j.bbagen.2011.06.005. 
      Epub 2011 Jun 25.