PMID- 21709671
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20230216
IS  - 1530-0307 (Electronic)
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 91
IP  - 10
DP  - 2011 Oct
TI  - Development and characterization of xenograft model systems for adenoid cystic 
      carcinoma.
PG  - 1480-90
LID - 10.1038/labinvest.2011.105 [doi]
AB  - Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in 
      human salivary glands, and it also arises in the glandular tissue of other organ 
      systems. To address the paucity of experimental model systems for this tumor 
      type, we have undertaken a program of transplanting tissue samples of human ACC 
      into immunodeficient nu/nu mice to create xenograft model systems. In 17 of 23 
      attempts (74%), xenograft tumors were successfully grown. In all cases, the 
      histologic appearance of the donating tumor was recapitulated in the subsequent 
      xenograft. Characterization of a subset of xenograft models by 
      immunohistochemical biomarkers and by RNA transcript microarray analysis showed 
      good fidelity in the recapitulation of gene expression patterns in the xenograft 
      tumors compared with the human donor tumors. As ACC is known to frequently 
      contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in 
      situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed 
      MYB locus break-apart and MYB-NFIB locus fusion. Of 12 xenograft models, 11 (92%) 
      revealed MYB locus rearrangement and 10 (83%) showed evidence of fusion of the 
      MYB and NFIB loci. The two related xenograft models (derived from primary and 
      metastatic tumors, respectively, of the same human subject) were karyotyped, 
      showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion 
      partner gene. Overall, our results indicate that ACC is amenable to xenografting 
      and that ACC xenograft models recapitulate the molecular and morphologic 
      characteristics of human tumors, suggesting utility as valid experimental and 
      preclinical model systems for this disease.
CI  - (c) 2011 USCAP, Inc All rights reserved
FAU - Moskaluk, Christopher A
AU  - Moskaluk CA
AD  - Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. 
      cam5p@virginia.edu
FAU - Baras, Alexander S
AU  - Baras AS
FAU - Mancuso, Stefani A
AU  - Mancuso SA
FAU - Fan, Hao
AU  - Fan H
FAU - Davidson, Robert J
AU  - Davidson RJ
FAU - Dirks, Dawn C
AU  - Dirks DC
FAU - Golden, Wendy L
AU  - Golden WL
FAU - Frierson, Henry F Jr
AU  - Frierson HF Jr
LA  - eng
GR  - P30 CA044579/CA/NCI NIH HHS/United States
GR  - R01 DE014694/DE/NIDCR NIH HHS/United States
GR  - RC1 DE020687/DE/NIDCR NIH HHS/United States
GR  - RC1-DE020687/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110627
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Biomarkers)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (NFIB protein, human)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Carcinoma, Adenoid Cystic/genetics/*metabolism/*pathology/secondary
MH  - Chromosome Mapping
MH  - *Disease Models, Animal
MH  - Gene Expression Profiling
MH  - Gene Fusion
MH  - Genes, myb
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Mice
MH  - Mice, Nude
MH  - Microarray Analysis
MH  - NFI Transcription Factors/genetics
MH  - Neoplasm Transplantation
MH  - RNA/metabolism
MH  - Salivary Gland Neoplasms/genetics/*metabolism/*pathology
MH  - Translocation, Genetic
MH  - *Transplantation, Heterologous
PMC - PMC4151120
MID - NIHMS560283
EDAT- 2011/06/29 06:00
MHDA- 2011/12/13 00:00
PMCR- 2014/09/02
CRDT- 2011/06/29 06:00
PHST- 2011/06/29 06:00 [entrez]
PHST- 2011/06/29 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2014/09/02 00:00 [pmc-release]
AID - S0023-6837(22)02715-5 [pii]
AID - 10.1038/labinvest.2011.105 [doi]
PST - ppublish
SO  - Lab Invest. 2011 Oct;91(10):1480-90. doi: 10.1038/labinvest.2011.105. Epub 2011 
      Jun 27.