PMID- 21711570
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20240109
IS  - 1743-7075 (Electronic)
IS  - 1743-7075 (Linking)
VI  - 8
IP  - 1
DP  - 2011 Jun 29
TI  - Cinnamon extract inhibits alpha-glucosidase activity and dampens postprandial glucose 
      excursion in diabetic rats.
PG  - 46
LID - 10.1186/1743-7075-8-46 [doi]
AB  - BACKGROUND: alpha-glucosidase inhibitors regulate postprandial hyperglycemia (PPHG) 
      by impeding the rate of carbohydrate digestion in the small intestine and thereby 
      hampering the diet associated acute glucose excursion. PPHG is a major risk 
      factor for diabetic vascular complications leading to disabilities and mortality 
      in diabetics. Cinnamomum zeylanicum, a spice, has been used in traditional 
      medicine for treating diabetes. In this study we have evaluated the alpha-glucosidase 
      inhibitory potential of cinnamon extract to control postprandial blood glucose 
      level in maltose, sucrose loaded STZ induced diabetic rats. METHODS: The methanol 
      extract of cinnamon bark was prepared by Soxhlet extraction. Phytochemical 
      analysis was performed to find the major class of compounds present in the 
      extract. The inhibitory effect of cinnamon extract on yeast alpha-glucosidase and 
      rat-intestinal alpha-glucosidase was determined in vitro and the kinetics of enzyme 
      inhibition was studied. Dialysis experiment was performed to find the nature of 
      the inhibition. Normal male Albino wistar rats and STZ induced diabetic rats were 
      treated with cinnamon extract to find the effect of cinnamon on postprandial 
      hyperglycemia after carbohydrate loading. RESULTS: Phytochemical analysis of the 
      methanol extract displayed the presence of tannins, flavonoids, glycosides, 
      terpenoids, coumarins and anthraquinones. In vitro studies had indicated 
      dose-dependent inhibitory activity of cinnamon extract against yeast 
      alpha-glucosidase with the IC 50 value of 5.83 mug/ml and mammalian alpha-glucosidase with 
      IC 50 value of 670 mug/ml. Enzyme kinetics data fit to LB plot pointed out 
      competitive mode of inhibition and the membrane dialysis experiment revealed 
      reversible nature of inhibition. In vivo animal experiments are indicative of 
      ameliorated postprandial hyperglycemia as the oral intake of the cinnamon extract 
      (300 mg/kg body wt.) significantly dampened the postprandial hyperglycemia by 
      78.2% and 52.0% in maltose and sucrose loaded STZ induced diabetic rats 
      respectively, compared to the control. On the other hand, in rats that received 
      glucose and cinnamon extract, postprandial hyperglycemia was not effectively 
      suppressed, which indicates that the observed postprandial glycemic amelioration 
      is majorly due to alpha-glucosidase inhibition. CONCLUSIONS: The current study 
      demonstrates one of the mechanisms in which cinnamon bark extract effectively 
      inhibits alpha-glucosidase leading to suppression of postprandial hyperglycemia in 
      STZ induced diabetic rats loaded with maltose, sucrose. This bark extract shows 
      competitive, reversible inhibition on alpha-glucosidase enzyme. Cinnamon extract 
      could be used as a potential nutraceutical agent for treating postprandial 
      hyperglycemia. In future, specific inhibitor has to be isolated from the crude 
      extract, characterized and therapeutically exploited.
FAU - Mohamed Sham Shihabudeen, H
AU  - Mohamed Sham Shihabudeen H
AD  - Structural Biology Lab, Centre for Biomedical Research, SBST, VIT University, 
      Vellore, India. m.kavitha@vit.ac.in.
FAU - Hansi Priscilla, D
AU  - Hansi Priscilla D
FAU - Thirumurugan, Kavitha
AU  - Thirumurugan K
LA  - eng
PT  - Journal Article
DEP - 20110629
PL  - England
TA  - Nutr Metab (Lond)
JT  - Nutrition & metabolism
JID - 101231644
PMC - PMC3155477
EDAT- 2011/06/30 06:00
MHDA- 2011/06/30 06:01
PMCR- 2011/06/29
CRDT- 2011/06/30 06:00
PHST- 2011/02/28 00:00 [received]
PHST- 2011/06/29 00:00 [accepted]
PHST- 2011/06/30 06:00 [entrez]
PHST- 2011/06/30 06:00 [pubmed]
PHST- 2011/06/30 06:01 [medline]
PHST- 2011/06/29 00:00 [pmc-release]
AID - 1743-7075-8-46 [pii]
AID - 10.1186/1743-7075-8-46 [doi]
PST - epublish
SO  - Nutr Metab (Lond). 2011 Jun 29;8(1):46. doi: 10.1186/1743-7075-8-46.