PMID- 21712071
OWN - NLM
STAT- MEDLINE
DCOM- 20120705
LR  - 20220321
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 190
DP  - 2011 Sep 8
TI  - Expression of monocyte chemoattractant protein-1 and its induction by tumor 
      necrosis factor receptor 1 in sensory neurons in the ventral rhizotomy model of 
      neuropathic pain.
PG  - 354-66
LID - 10.1016/j.neuroscience.2011.06.036 [doi]
AB  - The expression and role of monocyte chemoattractant protein-1 (MCP-1) in the rat 
      dorsal root ganglion (DRG) and spinal cord was evaluated in the lumbar 5 ventral 
      rhizotomy (L5 VR) model of neuropathic pain. MCP-1 protein expression in the 
      L4/L5 DRG neurons following L5 VR peaked after 3 days, and then declined. 
      Immunohistochemistry showed that no MCP-1 immunoreactivity was observed in the 
      spinal cord after L5 VR, while enzyme-linked immunosorbent assay (ELISA) revealed 
      a small but significant increase in MCP-1 protein content. L5 VR resulted in 
      robust and prolonged mechanical allodynia and thermal hyperalgesia. 
      Administration of anti-MCP-1 neutralizing antibody before and at early time 
      points after L5 VR resulted in a significant attenuation of mechanical allodynia 
      and thermal hyperalgesia, while post-treatment had a weaker effect on established 
      neuropathic pain. Extensive colocalization of tumor necrosis factor receptor 1 
      (TNFR1) and MCP-1 was observed in the L5 DRG following L5 VR, and treatment with 
      TNFR1 antisense oligonucleotide reduced L5 VR-induced MCP-1 expression in L5 DRG 
      neurons and neuropathic pain behaviors. MCP-1/chemokine (C-C motif) receptor 2 
      signaling has been proposed as a major regulator of macrophage trafficking. In 
      contrast to the effect on pain behaviors, however, intrathecal administration of 
      anti-MCP-1 neutralizing antibody had no effect on the L5 VR-induced increase in 
      ED-1-immunoreactive macrophages in the L5 DRG and the distal stump of the 
      transected L5 ventral root. These data indicate that increased MCP-1 in DRG 
      neurons might participate in the initiation, rather than the maintenance, of 
      neuropathic pain induced by L5 VR. Furthermore, increased MCP-1 in the DRG is 
      induced by TNF-alpha/TNFR1 and has no effect on the infiltration of macrophages into 
      the DRG following L5 VR.
CI  - Copyright (c) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Jeon, S-M
AU  - Jeon SM
AD  - Department of Anatomy, School of Medicine, Kyungpook National University, 2-101, 
      Dongin Dong, Daegu 700-422, South Korea.
FAU - Sung, J-K
AU  - Sung JK
FAU - Cho, H-J
AU  - Cho HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110625
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*metabolism
MH  - Disease Models, Animal
MH  - Ganglia, Spinal/*metabolism/physiopathology
MH  - Hyperalgesia/metabolism/physiopathology
MH  - Male
MH  - Neuralgia/*metabolism/physiopathology
MH  - Physical Stimulation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Tumor Necrosis Factor, Type I/*metabolism
MH  - Rhizotomy
MH  - Sensory Receptor Cells/*metabolism
MH  - Spinal Cord/*metabolism/physiopathology
EDAT- 2011/06/30 06:00
MHDA- 2012/07/06 06:00
CRDT- 2011/06/30 06:00
PHST- 2010/10/22 00:00 [received]
PHST- 2011/06/07 00:00 [revised]
PHST- 2011/06/11 00:00 [accepted]
PHST- 2011/06/30 06:00 [entrez]
PHST- 2011/06/30 06:00 [pubmed]
PHST- 2012/07/06 06:00 [medline]
AID - S0306-4522(11)00720-2 [pii]
AID - 10.1016/j.neuroscience.2011.06.036 [doi]
PST - ppublish
SO  - Neuroscience. 2011 Sep 8;190:354-66. doi: 10.1016/j.neuroscience.2011.06.036. 
      Epub 2011 Jun 25.