PMID- 21716731
OWN - NLM
STAT- MEDLINE
DCOM- 20111027
LR  - 20211020
IS  - 1740-2530 (Electronic)
IS  - 1740-2522 (Print)
IS  - 1740-2522 (Linking)
VI  - 2011
DP  - 2011
TI  - NOD dendritic cells stimulated with Lactobacilli preferentially produce IL-10 
      versus IL-12 and decrease diabetes incidence.
PG  - 630187
LID - 10.1155/2011/630187 [doi]
LID - 630187
AB  - Dendritic cells (DCs) from NOD mice produced high levels of IL-12 that induce 
      IFNgamma-producing T cells involved in diabetes development. We propose to utilize 
      the microorganism ability to induce tolerogenic DCs to abrogate the 
      proinflammatory process and prevent diabetes development. NOD DCs were stimulated 
      with Lactobacilli (nonpathogenic bacteria targeting TLR2) or lipoteichoic acid 
      (LTA) from Staphylococcus aureus (TLR2 agonist). LTA-treated DCs produced much 
      more IL-12 than IL-10 and accelerated diabetes development when transferred into 
      NOD mice. In contrast, stimulation of NOD DCs with L. casei favored the 
      production of IL-10 over IL-12, and their transfer decreased disease incidence 
      which anti-IL-10R antibodies restored. These data indicated that L. casei can 
      induce NOD DCs to develop a more tolerogenic phenotype via production of the 
      anti-inflammatory cytokine, IL-10. Evaluation of the relative production of IL-10 
      and IL-12 by DCs may be a very useful means of identifying agents that have 
      therapeutic potential.
FAU - Manirarora, Jean N
AU  - Manirarora JN
AD  - Department of Microbiology and Immunology, University of Louisville HSC, 319 
      Abraham Flexner Way, Louisville, KY 40202, USA.
FAU - Parnell, Sarah A
AU  - Parnell SA
FAU - Hu, Yoon-Hyeon
AU  - Hu YH
FAU - Kosiewicz, Michele M
AU  - Kosiewicz MM
FAU - Alard, Pascale
AU  - Alard P
LA  - eng
GR  - R56 DK075892/DK/NIDDK NIH HHS/United States
GR  - R56DK075892/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110612
PL  - Egypt
TA  - Clin Dev Immunol
JT  - Clinical & developmental immunology
JID - 101183692
RN  - 0 (Ligands)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/cytology/*immunology
MH  - Diabetes Mellitus/epidemiology/*immunology
MH  - Female
MH  - Incidence
MH  - Interleukin-10/*biosynthesis/immunology
MH  - Interleukin-12/*biosynthesis/immunology
MH  - Lactobacillus/*physiology
MH  - Ligands
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred NOD
MH  - Phenotype
PMC - PMC3119457
EDAT- 2011/07/01 06:00
MHDA- 2011/10/28 06:00
PMCR- 2011/06/12
CRDT- 2011/07/01 06:00
PHST- 2011/01/15 00:00 [received]
PHST- 2011/03/25 00:00 [revised]
PHST- 2011/04/05 00:00 [accepted]
PHST- 2011/07/01 06:00 [entrez]
PHST- 2011/07/01 06:00 [pubmed]
PHST- 2011/10/28 06:00 [medline]
PHST- 2011/06/12 00:00 [pmc-release]
AID - 10.1155/2011/630187 [doi]
PST - ppublish
SO  - Clin Dev Immunol. 2011;2011:630187. doi: 10.1155/2011/630187. Epub 2011 Jun 12.