PMID- 2172014
OWN - NLM
STAT- MEDLINE
DCOM- 19901213
LR  - 20190620
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 272
IP  - 1-2
DP  - 1990 Oct 15
TI  - Fluoride activates diradylglycerol and superoxide generation in human neutrophils 
      via PLD/PA phosphohydrolase-dependent and -independent pathways.
PG  - 19-24
AB  - In contrast to the rapid, ethanol-inhibited superoxide generation by the 
      receptor-linked agonist formyl-methionyl-leucyl-phenylalanine (fMLP), 
      fluoride-activated superoxide generation occurs after a prolonged lag, and as 
      shown herein is relatively ethanol-insensitive. We have investigated 
      fluoride-activation of diradylglycerol generation and phospholipase D activity. 
      Fluoride induces a very large increase in diradylglycerol mass (both 
      1,2-diacylglycerol (DAG) and 1-O-alkyl,2-acylglycerol (EAG)), with kinetics 
      similar to superoxide generation. Unlike fMLP-activated diglyceride generation 
      which is completely inhibited by ethanol, that produced by fluoride is only 
      partially (30%) blocked. When the phosphatidylcholine pool is 3H-prelabeled, 
      fluoride activates both [3H]phosphatidic acid (PA) and [3H]diglyceride generation 
      with similar kinetics. Partial inhibition of the production of these species by 
      ethanol was seen, coincident with the appearance of [3H]phosphatidylethanol, 
      indicating phospholipase D-dependent transphosphatidylation had occurred. The 
      data are consistent with the fluoride activation of PA and diglyceride generation 
      by both phospholipase D-dependent and -independent (presumably phospholipase C) 
      mechanisms.
FAU - Olson, S C
AU  - Olson SC
AD  - Department of Biochemistry, Emory University Medical School, Atlanta, GA 30322.
FAU - Tyagi, S R
AU  - Tyagi SR
FAU - Lambeth, J D
AU  - Lambeth JD
LA  - eng
GR  - AI122809/AI/NIAID NIH HHS/United States
GR  - CA46508/CA/NCI NIH HHS/United States
GR  - T32 DK07298/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (1,2-diacylglycerol)
RN  - 0 (Diglycerides)
RN  - 0 (Phosphatidic Acids)
RN  - 0 (Phosphatidylethanolamines)
RN  - 11062-77-4 (Superoxides)
RN  - 3K9958V90M (Ethanol)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 82231-61-6 (diarachidonyl diglyceride)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 3.1.3.4 (Phosphatidate Phosphatase)
RN  - EC 3.1.4.4 (Phospholipase D)
SB  - IM
MH  - Diglycerides/*blood
MH  - Ethanol/pharmacology
MH  - Kinetics
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*metabolism
MH  - Phosphatidate Phosphatase/*blood
MH  - Phosphatidic Acids/blood
MH  - Phosphatidylethanolamines/blood
MH  - Phospholipase D/*blood
MH  - Sodium Fluoride/*pharmacology
MH  - Superoxides/*blood
EDAT- 1990/10/15 00:00
MHDA- 1990/10/15 00:01
CRDT- 1990/10/15 00:00
PHST- 1990/10/15 00:00 [pubmed]
PHST- 1990/10/15 00:01 [medline]
PHST- 1990/10/15 00:00 [entrez]
AID - 0014-5793(90)80439-P [pii]
AID - 10.1016/0014-5793(90)80439-p [doi]
PST - ppublish
SO  - FEBS Lett. 1990 Oct 15;272(1-2):19-24. doi: 10.1016/0014-5793(90)80439-p.