PMID- 2172295
OWN - NLM
STAT- MEDLINE
DCOM- 19901213
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 28
IP  - 9
DP  - 1990 Sep
TI  - Identification of a nucleocapsid protein as a specific serological marker of 
      human herpesvirus 6 infection.
PG  - 1957-62
AB  - Enveloped whole virions and nucleocapsids of human herpesvirus 6 (HHV-6) strain 
      Z29 were purified from supernatant fluids of infected human cord blood 
      lymphocytes by filtration through polyvinylpyrrolidone-treated filters, banding 
      on a Nycondenz step gradient, and centrifugation through two successive 
      continuous sucrose gradients. More than 20 proteins ranging in molecular weight 
      from less than 30,000 to more than 200,000 were identified in preparations of 
      purified whole virions labeled with [35S]methionine and [35S]cysteine. 
      Immunogenic virion proteins of HHV-6 were identified in immunoblot assays with 
      human immune sera, immune sera generated from mice immunized with purified whole 
      virions or purified nucleocapsids, and a monoclonal antibody generated from a 
      mouse immunized with purified nucleocapsids. The sera and the monoclonal antibody 
      reacted strongly with a 101-kilodalton protein in the immunoblots, suggesting 
      that the protein is a component of the nucleocapsid. Human sera lacking 
      HHV-6-specific antibodies and seropositive for one or more of the other human 
      herpesviruses failed to react with this protein, indicating that it is a specific 
      serologic marker for HHV-6 infection.
FAU - Yamamoto, M
AU  - Yamamoto M
AD  - Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, 
      Georgia 30333.
FAU - Black, J B
AU  - Black JB
FAU - Stewart, J A
AU  - Stewart JA
FAU - Lopez, C
AU  - Lopez C
FAU - Pellett, P E
AU  - Pellett PE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Biomarkers)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral
MH  - Biomarkers
MH  - Capsid/*immunology/isolation & purification
MH  - Herpesviridae Infections/diagnosis/*immunology
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Humans
MH  - Serologic Tests
MH  - Viral Core Proteins/*immunology/isolation & purification
PMC - PMC268086
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
PMCR- 1990/09/01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
PHST- 1990/09/01 00:00 [pmc-release]
AID - 10.1128/jcm.28.9.1957-1962.1990 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1990 Sep;28(9):1957-62. doi: 10.1128/jcm.28.9.1957-1962.1990.