PMID- 21724191
OWN - NLM
STAT- MEDLINE
DCOM- 20120109
LR  - 20151119
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1218
IP  - 42
DP  - 2011 Oct 21
TI  - Analyses of sulfonamide antibiotics in meat samples by on-line concentration 
      capillary electrochromatography-mass spectrometry.
PG  - 7640-7
LID - 10.1016/j.chroma.2011.06.027 [doi]
AB  - In this work, a series of poly(divinylbenzene-alkyl methacrylate) monolithic 
      stationary phases, which were prepared by single step in situ polymerization of 
      divinylbenzene and various alkyl methacrylates (butyl-, octyl-, lauryl- or 
      stearyl methacrylate), were developed as separation columns of nine common 
      sulfonamide antibiotics for capillary electrochromatography (CEC) coupled to mass 
      spectrometry (MS). Results indicated that the sulfonamide's retention became weak 
      with increased carbon chain length of alkyl methacrylate monomer (for example, 
      t(R)=68 min and 21 min for butyl- and lauryl methacrylate, respectively). Among 
      them, the poly(divinylbenzene-octyl methacrylate) (poly(DVB-OMA)) monolith was 
      regarded as the optimal separation column as this provided better resolution 
      within the shortest retention time. Moreover, the cross-sectional roughness of 
      the monolithic column-end, that was used to couple to the ESI interface, strongly 
      influenced the electrospray stability in the CEC-MS. Before the column was 
      connected to the ESI-MS, a simple polishing was done to reduce the roughness of 
      the column end that resulted to a great improvement in the signal stability. The 
      relative standard deviations (RSDs) of the peak areas for the unpolished and 
      polished ends of the poly(DVB-OMA) columns (n=5) were in the range of 46.1-60.2% 
      and 8.9-16.4%, respectively. Furthermore, optimization of the mobile phase 
      composition and the gradient elution strategy successfully determined the 
      sulfonamide antibiotics in meat samples with as low as 10 mug/L level.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Cheng, Yi-Jie
AU  - Cheng YJ
AD  - Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan 
      Christian University, 200 Chung Pei Road, Chung-Li 320, Taiwan.
FAU - Huang, Sing-Hao
AU  - Huang SH
FAU - Singco, Brenda
AU  - Singco B
FAU - Huang, Hsi-Ya
AU  - Huang HY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110617
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Methacrylates)
RN  - 0 (Polyvinyls)
RN  - 0 (Sulfonamides)
RN  - 0 (Vinyl Compounds)
RN  - IZ715T4SBU (divinyl benzene)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/chemistry
MH  - Capillary Electrochromatography/instrumentation/*methods
MH  - Drug Residues/*analysis/chemistry
MH  - Kidney
MH  - Liver
MH  - Mass Spectrometry/*methods
MH  - Meat/*analysis
MH  - Methacrylates
MH  - Polyvinyls
MH  - Sensitivity and Specificity
MH  - Sulfonamides/*analysis/chemistry
MH  - Surface Properties
MH  - Swine
MH  - Vinyl Compounds
EDAT- 2011/07/05 06:00
MHDA- 2012/01/10 06:00
CRDT- 2011/07/05 06:00
PHST- 2011/03/30 00:00 [received]
PHST- 2011/05/31 00:00 [revised]
PHST- 2011/06/07 00:00 [accepted]
PHST- 2011/07/05 06:00 [entrez]
PHST- 2011/07/05 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
AID - S0021-9673(11)00839-9 [pii]
AID - 10.1016/j.chroma.2011.06.027 [doi]
PST - ppublish
SO  - J Chromatogr A. 2011 Oct 21;1218(42):7640-7. doi: 10.1016/j.chroma.2011.06.027. 
      Epub 2011 Jun 17.