PMID- 21725926
OWN - NLM
STAT- MEDLINE
DCOM- 20120807
LR  - 20120322
IS  - 1724-6059 (Electronic)
IS  - 1121-8428 (Linking)
VI  - 25
IP  - 2
DP  - 2012 Mar-Apr
TI  - Increased apoptosis and expression of FasL, Bax and caspase-3 in human lupus 
      nephritis class II and IV.
PG  - 255-61
LID - 10.5301/JN.2011.8451 [doi]
AB  - BACKGROUND: Apoptosis is involved in glomerular injuries leading to 
      glomerulonephritis. However, the role of renal cellular apoptosis in the 
      pathogenesis and progression of human lupus nephritis (LN) is controversial, and 
      studies on the expression of apoptosis-related proteins, such as FasL, Bax and 
      caspase-3, in different classifications of human LN renal tissues are limited. 
      METHODS: Thirty-two samples of LN tissues, including 10 cases of class II and 22 
      cases of class IV LN, and 5 cases of human normal renal tissues were obtained. 
      Expression of FasL, Bax and caspase-3 proteins in LN tissues was examined by 
      immunohistochemical staining. Apoptotic cells were evaluated by the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. 
      RESULTS: Expression of FasL, Bax and caspase-3 and TUNEL-positive cells in 
      glomerular parenchymal cells, renal tubular epithelial cells and interstitial 
      inflammatory cells were higher in LN tissues compared with controls. Expression 
      of Bax and caspase-3, but not FasL, was significantly higher in glomeruli of 
      class IV LN than those of class II LN. The apoptotic cell count per glomerulus 
      was significantly higher in class IV LN than class II LN (p<0.05). CONCLUSIONS: 
      Increased apoptosis and the expression of FasL, Bax and caspase-3 in human LN 
      suggest that apoptosis might be induced through pathways of these proteins in the 
      pathogenesis process and play an important role in LN progression through Bax and 
      caspase-3, but not FasL.
FAU - Cui, Ji-Hong
AU  - Cui JH
AD  - Department of Pathology, State Key Laboratory of Cancer Biology, Xijing Hospital, 
      Fourth Military Medical University, Xi'an, Shaanxi, PR China.
FAU - Qiao, Qing
AU  - Qiao Q
FAU - Guo, Ying
AU  - Guo Y
FAU - Zhang, Ya-Qing
AU  - Zhang YQ
FAU - Cheng, Hong
AU  - Cheng H
FAU - He, Fu-Rong
AU  - He FR
FAU - Zhang, Jing
AU  - Zhang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - J Nephrol
JT  - Journal of nephrology
JID - 9012268
RN  - 0 (BAX protein, human)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Apoptosis
MH  - Caspase 3/*analysis/physiology
MH  - Fas Ligand Protein/*analysis/physiology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Glomerulus/pathology
MH  - Kidney Tubules/chemistry/pathology
MH  - Lupus Nephritis/metabolism/*pathology
MH  - Male
MH  - Middle Aged
MH  - bcl-2-Associated X Protein/*analysis/physiology
EDAT- 2011/07/05 06:00
MHDA- 2012/08/08 06:00
CRDT- 2011/07/05 06:00
PHST- 2011/04/08 00:00 [accepted]
PHST- 2011/07/05 06:00 [entrez]
PHST- 2011/07/05 06:00 [pubmed]
PHST- 2012/08/08 06:00 [medline]
AID - 9C5E7C7E-0757-4043-960C-178DAF388D98 [pii]
AID - 10.5301/JN.2011.8451 [doi]
PST - ppublish
SO  - J Nephrol. 2012 Mar-Apr;25(2):255-61. doi: 10.5301/JN.2011.8451.