PMID- 21726155
OWN - NLM
STAT- MEDLINE
DCOM- 20111031
LR  - 20191210
IS  - 1532-4109 (Electronic)
IS  - 0360-1234 (Linking)
VI  - 46
IP  - 6
DP  - 2011
TI  - Determination of acibenzolar-S-methyl and its acidic metabolite in soils by 
      HPLC-diode array detection.
PG  - 550-6
LID - 10.1080/03601234.2011.583881 [doi]
AB  - A simple and accurate method for the analysis of acibenzolar-S-methyl 
      (benzo[1,2,3]thiadiazole-7-carbothioic acid-S-methyl ester; CGA 245 704; ASM) and 
      its major conversion product, benzo[1,2,3]thiadiazole-7-carboxylic acid (CGA 210 
      007; BTC), in soils is presented. ASM extraction from soil samples was performed 
      using acetonitrile and BTC was extracted with a mixture of potassium phosphate 
      buffer (0.5 M, pH 3) and acetonitrile (70:30 %, v/v). Both extracts were directly 
      analyzed in a high-performance liquid chromatography-diode array detection 
      (HPLC-DAD) system. Pesticide separation was achieved on a C18 (4.6 mm x 150 mm, 5 
      mum) analytical column with a isocratic elution of acetonitrile:water 40:60 % 
      (v/v) with 0.6 mL L(-)(1) acetic acid at a flow rate of 1 mL min(-)(1). Linear regression 
      coefficients (r (2)) of the external calibration curves were always above 0.9997. 
      The limits of detection (LOD) and quantification (LOQ) of the method were 0.005 
      and 0.02 mg kg(-)(1) for ASM, and 0.01 and 0.05 mg kg(-)(1) for BTC, respectively. 
      Recoveries were investigated at six fortification levels and were in the range of 
      90-120 % for ASM and 74-96 % for BTC with relative standard deviations (RSDs) 
      below 11 % in all cases. The method was also validated by analyzing freshly 
      spiked soil samples with 2.7% organic matter content at 0.5 mg kg(-)(1) level, with 
      slightly lower recovery values only for ASM. Moreover, recoveries for 
      intermediate aged residues of the analytes were similar to fresh residues. This 
      method was also applied to determine ASM half-life (t((1/2)) = 8.7 h) and the rate of 
      the acidic metabolite formation.
FAU - Myresiotis, Charalampos K
AU  - Myresiotis CK
AD  - Pesticide Science Laboratory, Aristotle University of Thessaloniki, Thessaloniki, 
      Greece.
FAU - Papadakis, Emmanouil N
AU  - Papadakis EN
FAU - Vryzas, Zisis G
AU  - Vryzas ZG
FAU - Papadopoulou-Mourkidou, Euphemia
AU  - Papadopoulou-Mourkidou E
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - J Environ Sci Health B
JT  - Journal of environmental science and health. Part. B, Pesticides, food 
      contaminants, and agricultural wastes
JID - 7607167
RN  - 0 (Pesticide Residues)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 0 (Thiadiazoles)
RN  - BCW6119347 (S-methyl benzo(1,2,3)thiadiazole-7-carbothioate)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Half-Life
MH  - Limit of Detection
MH  - Pesticide Residues/*analysis
MH  - Soil/*analysis
MH  - Soil Pollutants/*analysis
MH  - Thiadiazoles/*analysis
EDAT- 2011/07/06 06:00
MHDA- 2011/11/01 06:00
CRDT- 2011/07/06 06:00
PHST- 2011/07/06 06:00 [entrez]
PHST- 2011/07/06 06:00 [pubmed]
PHST- 2011/11/01 06:00 [medline]
AID - 10.1080/03601234.2011.583881 [doi]
PST - ppublish
SO  - J Environ Sci Health B. 2011;46(6):550-6. doi: 10.1080/03601234.2011.583881.